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Neuroprotection of Cytisine Against Cerebral Ischemia–Reperfusion Injury in Mice by Regulating NR2B-ERK/CREB Signal Pathway
- Source :
- Neurochemical Research. 43:1575-1586
- Publication Year :
- 2018
- Publisher :
- Springer Science and Business Media LLC, 2018.
-
Abstract
- The aim of the study was to elucidate the therapeutic effects of Cytisine (CYT) on cerebral ischemia-reperfusion injury in mice. Male ICR mice were pretreated with reagents (drug), and then subjected to 2 h focal cerebral ischemia and 24 h reperfusion. Morphologically, the histopathological impairment were estimated by the TTC, HE and TUNEL staining. The expression of GluN2B-containing NMDA receptor, phosphorylation of extracellular regulated protein kinases, total ERK, phosphorylation of cAMP-response element binding protein and total CREB were determined by immunofluorescence and Western blot assay, respectively. The mRNA expression of NR2B, ERK and CREB were quantified by the real-time RT-PCR. CYT significantly diminished the infarct size and neuronal apoptosis. Additionally, it ameliorated histopathological lesion dramatically. CYT promoted the phosphorylation of ERK, CREB and their mRNA expression. In contrast, the expression of NR2B was suppressed in concomitant with the down-regulation of genes. The overall results thus far suggest that CYT confers the neuroprotection against cerebral I/R injury by regulating the NR2B-ERK/CREB signal pathway.
- Subjects :
- Male
0301 basic medicine
MAPK/ERK pathway
MAP Kinase Signaling System
Pharmacology
CREB
Receptors, N-Methyl-D-Aspartate
Biochemistry
Neuroprotection
Brain Ischemia
Mice
03 medical and health sciences
Cellular and Molecular Neuroscience
Cytisine
chemistry.chemical_compound
Alkaloids
0302 clinical medicine
medicine
Animals
Cyclic AMP Response Element-Binding Protein
Mice, Inbred ICR
TUNEL assay
biology
Kinase
General Medicine
medicine.disease
Azocines
030104 developmental biology
chemistry
Reperfusion Injury
biology.protein
Phosphorylation
Reperfusion injury
Quinolizines
030217 neurology & neurosurgery
Signal Transduction
Subjects
Details
- ISSN :
- 15736903 and 03643190
- Volume :
- 43
- Database :
- OpenAIRE
- Journal :
- Neurochemical Research
- Accession number :
- edsair.doi.dedup.....902b874c2f68a6f91a2d5f65c16e30b6