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Organic two-photon nanoparticles modulate reactive oxygen species, intracellular calcium concentration, and mitochondrial membrane potential during apoptosis of human gastric carcinoma SGC-7901 cells
- Source :
- Biotechnology letters. 38(8)
- Publication Year :
- 2016
-
Abstract
- To investigate the biocompatibility of human gastric carcinoma cells (SGC-7901) with organic two-photon nanoparticles (NPs).Different concentrations of NPs were incubated with SGC-7901 cells for different times. The levels of cell apoptosis, reactive oxygen species (ROS), intracellular calcium, and mitochondrial membrane potential (MMP) were measured by staining the SGC-7901 cells with Annexin V-FITC/PI, 2',7'-dichlorofluorescin diacetate, Fluo-3 AM, and Rhodamine 123, followed by the flow cytometry assay. NPs at4 µg/ml, did not have any significant effect on apoptosis, necrosis, generation of ROS, increase of intracellular Ca(2+) concentration or decrease of MMP in SGC-7901 cells, but4 µg/ml had a major effects on all the above mentioned parameters.2,5,2',5'-Tetra(4-N,N-diphenylamine styryl) biphenyl NPs can be used at an appropriate concentration as a safe drug carrier or imaging marker and may serve as an effective tool for developing a photodynamic cancer therapy.
- Subjects :
- inorganic chemicals
0301 basic medicine
chemistry.chemical_element
Bioengineering
Apoptosis
02 engineering and technology
Calcium
Biology
Applied Microbiology and Biotechnology
Rhodamine 123
Calcium in biology
Flow cytometry
03 medical and health sciences
chemistry.chemical_compound
Annexin
Stomach Neoplasms
Cell Line, Tumor
medicine
Humans
chemistry.chemical_classification
Membrane Potential, Mitochondrial
Reactive oxygen species
medicine.diagnostic_test
General Medicine
021001 nanoscience & nanotechnology
030104 developmental biology
chemistry
Biochemistry
Nanoparticles
0210 nano-technology
Drug carrier
Reactive Oxygen Species
Biotechnology
Subjects
Details
- ISSN :
- 15736776
- Volume :
- 38
- Issue :
- 8
- Database :
- OpenAIRE
- Journal :
- Biotechnology letters
- Accession number :
- edsair.doi.dedup.....907eab5424169227c6192212c3fdef8b