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miR-190-5p Alleviates Myocardial Ischemia-Reperfusion Injury by Targeting PHLPP1
miR-190-5p Alleviates Myocardial Ischemia-Reperfusion Injury by Targeting PHLPP1
- Source :
- Disease Markers, Vol 2021 (2021), Disease Markers
- Publication Year :
- 2021
- Publisher :
- Hindawi Limited, 2021.
-
Abstract
- Objective. Myocardial ischemia-reperfusion (I/R) injury (MIRI) refers to the more serious myocardial injury after blood flow recovery, which seriously affects the prognosis of patients with ischemic cardiomyopathy. This study explored the new targets for MIRI treatment by investigating the effects of miR-190-5p and its downstream target on the structure and function of myocardial cells. Methods. We injected agomir miR-190-5p into the tail vein of rats to increase the expression of miR-190-5p in rat myocardial cells and made an I/R rat model by coronary artery occlusion. We used 2,3,5-triphenyl tetrazolium chloride staining, lactate dehydrogenase (LDH) detection, echocardiography, and hematoxylin-eosin (HE) staining to determine the degree of myocardial injury in I/R rats. In addition, we detected the expression of inflammatory factors and apoptosis-related molecules in rat serum and myocardial tissue to determine the level of inflammation and apoptosis in rat myocardium. Finally, we determined the downstream target of miR-190-5p by Targetscan system and dual luciferase reporter assay. Results. The expression of miR-190-5p in an I/R rat myocardium was significantly lower than that in normal rats. After treatment of I/R rats with agomir miR-190-5p, the ischemic area of rat myocardium and the concentration of LDH decreased. The results of echocardiography and HE staining also found that overexpression of miR-190-5p improved the structure and function of rat myocardium. miR-190-5p was also found to improve the viability of H9c2 cells in vitro and reduce the level of apoptosis of H9c2 cells. The results of Targetscan system and dual luciferase reporter assay found that miR-190-5p targeted to inhibit pleckstrin homology domain leucine-rich repeat protein phosphatase 1 (PHLPP1). In addition, inhibition of PHLPP1 was found to improve the viability of H9c2 cells. Conclusion. Therefore, miR-190-5p can reduce the inflammation and apoptosis of myocardium by targeting PHLPP1, thereby alleviating MIRI.
- Subjects :
- Male
Medicine (General)
medicine.medical_specialty
Myocardial ischemia
Article Subject
Clinical Biochemistry
Apoptosis
Myocardial Reperfusion Injury
Cell Line
Rats, Sprague-Dawley
R5-920
Internal medicine
Genetics
Animals
Medicine
Molecular Biology
L-Lactate Dehydrogenase
business.industry
Myocardium
Biochemistry (medical)
Nuclear Proteins
General Medicine
medicine.disease
Rats
Disease Models, Animal
MicroRNAs
Echocardiography
Cardiology
Inflammation Mediators
business
Reperfusion injury
Research Article
Subjects
Details
- ISSN :
- 18758630 and 02780240
- Volume :
- 2021
- Database :
- OpenAIRE
- Journal :
- Disease Markers
- Accession number :
- edsair.doi.dedup.....909438b9e232036a26d0a4ae08cded91
- Full Text :
- https://doi.org/10.1155/2021/8709298