Back to Search
Start Over
Biosynthesis and secretion of Iutropin in rat gonadotropes treated with swainsonine
- Source :
- Acta Endocrinologica. 119:132-138
- Publication Year :
- 1988
- Publisher :
- Oxford University Press (OUP), 1988.
-
Abstract
- Biosynthesis and secretion of LH were investigated using primary cultures of rat gonadotropes following exposure to swainsonine, an inhibitor of N-linked oligosaccharide processing. The inhibition of terminal glycosylation by swainsonine was assessed by the increase in incorporation of [3H]mannose and the decrease of incorporation of [3H]galactose into the trichloroacetic acid-insoluble and anti-ovine LHβ immunoprecipitable fractions. This inhibitor led to an increase in cellular LH with high affinity for concanavalin A and a decrease of LH with less affinity for the lectin, although the cellular LH was not decreased. The swainsonine-treated cells accumulated highly basic LH (pI, 9.6, 10.0) in a time-dependent manner. Although the treated cells showed a decrease in the responsiveness to hormonal stimulation, there were no differenes in the rate and extent of LH secretion induced by maximal stimulation with 100 nmol/I GnRH between the control and treated cells over 24 h. The accumulation of highly basic LH molecules did not result from a decrease in their secretion, since they were secreted in the presence and absence of GnRH. It is concluded that significant changes in N-linked oligosaccharide structure of LH have no effect upon its secretion.
- Subjects :
- Male
medicine.medical_specialty
Endocrinology, Diabetes and Metabolism
Mannose
Stimulation
Chemical Fractionation
Gonadotropic cell
Chromatography, Affinity
chemistry.chemical_compound
Alkaloids
Endocrinology
Pituitary Gland, Anterior
Internal medicine
medicine
Animals
Secretion
Cells, Cultured
chemistry.chemical_classification
biology
Swainsonine
Lectin
Rats, Inbred Strains
General Medicine
Luteinizing Hormone
Oligosaccharide
Rats
chemistry
Concanavalin A
biology.protein
Isoelectric Focusing
Orchiectomy
Pituitary Hormone-Releasing Hormones
Subjects
Details
- ISSN :
- 1479683X and 08044643
- Volume :
- 119
- Database :
- OpenAIRE
- Journal :
- Acta Endocrinologica
- Accession number :
- edsair.doi.dedup.....90a2ede7cfee2b670afaf40ece698dd9