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ZmLEA3, a Multifunctional Group 3 LEA Protein from Maize (Zea mays L.), is Involved in Biotic and Abiotic Stresses
- Source :
- Plant and Cell Physiology. 54:944-959
- Publication Year :
- 2013
- Publisher :
- Oxford University Press (OUP), 2013.
-
Abstract
- Late embryogenesis abundant (LEA) proteins accumulate to high levels during the late stage of seed maturation and in response to water deficit, and are involved in protecting higher plants from damage caused by environmental stresses, especially drought. In the present study, a novel maize (Zea mays L.) group 3 LEA gene, ZmLEA3, was identified and later characterized using transgenic tobacco plants to investigate its functions in abiotic and biotic stresses. Transcript accumulation demonstrated that ZmLEA3 was induced in leaves by high salinity, low temperature, osmotic and oxidative stress as well as by signaling molecules such as ABA, salicylic acid (SA) and methyl jasmonate (MeJA). The transcript of ZmLEA3 could also be induced by pathogens [Pseudomonas syringae pv. tomato DC3000 (pst dc3000)]. ZmLEA3 is located in the cytosol and the nucles. Further study indicated that the ZmLEA3 protein could bind Mn(2+), Fe(3+), Cu(2+) and Zn(2+). Overexpression of ZmLEA3 in transgenic tobacco (Nicotiana tabacum) and yeast (GS115) conferred tolerance to osmotic and oxidative stresses. Interestingly, we also found that overexpression of ZmLEA3 in transgenic tobacco increased the hypersensitive cell death triggered by pst dc3000 and enhanced the expression of PR1a, PR2 and PR4 when compared with the wild type. Thus, we proposed that the ZmLEA3 protein plays a role in protecting plants from damage by protecting protein structure and binding metals under osmotic and oxidative stresses. In addition, ZmLEA3 may also enhance transgenic plant tolerance to biotic stress.
- Subjects :
- Hypersensitive response
Osmotic shock
Physiology
Recombinant Fusion Proteins
Nicotiana tabacum
Green Fluorescent Proteins
Molecular Sequence Data
Pseudomonas syringae
Plant Science
medicine.disease_cause
Zea mays
Chromatography, Affinity
chemistry.chemical_compound
Gene Expression Regulation, Plant
Osmotic Pressure
Stress, Physiological
Tobacco
Botany
medicine
Amino Acid Sequence
RNA, Messenger
Plant Proteins
Methyl jasmonate
L-Lactate Dehydrogenase
biology
fungi
Computational Biology
food and beverages
Cell Biology
General Medicine
Biotic stress
Plants, Genetically Modified
biology.organism_classification
Adaptation, Physiological
Cell biology
Plant Leaves
Oxidative Stress
Protein Transport
chemistry
Metals
Oxidation-Reduction
Sequence Alignment
Oxidative stress
Salicylic acid
Protein Binding
Subcellular Fractions
Subjects
Details
- ISSN :
- 14719053 and 00320781
- Volume :
- 54
- Database :
- OpenAIRE
- Journal :
- Plant and Cell Physiology
- Accession number :
- edsair.doi.dedup.....90c3a649942db6e79079934e0042a7fa