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A rapid and rapid method to quantify poly (γ-glutamic acid) content via copper ion complexation

Authors :
Mengmeng Wang
Li Wang
Yipeng Zang
Chenrui Yu
Dandan Liu
Yalan Ding
Wenjin Yue
Guangjun Nie
Source :
International Journal of Biological Macromolecules. 180:411-417
Publication Year :
2021
Publisher :
Elsevier BV, 2021.

Abstract

Presently, there have been some limitations in most of methods to determine poly (γ-glutamic acid) (γ-PGA) content because of many impurities in test specimens. It is necessary to establish a rapid and accurate method to quantify γ-PGA content. In this work, γ-PGA and some impurities commonly seen in fermented broth like glucose, glutamic acid and proteins were used to complex with copper ions. The results show that only γ-PGA can make copper ion precipitated, which content linearly correlates with the precipitate amount. From the study on the validity of the method, it is found that the accuracy and precision are 95.82% and 99.29%, much higher than the ones of method UV and weighing. Therefore, the method via the complexation of copper ion will be popularized to determine γ-PGA content in crude biological samples.

Details

ISSN :
01418130
Volume :
180
Database :
OpenAIRE
Journal :
International Journal of Biological Macromolecules
Accession number :
edsair.doi.dedup.....90e80497a3d785f47402abfa6cad0d26