Additional file 1 of ACBD3 modulates KDEL receptor interaction with PKA for its trafficking via tubulovesicular carrier

Additional file 1: Figure S1. (A) Characterization of HeLa cells expressing KDELR1 tagged with myc-BirA*. HeLa cells expressing KDELR1-myc-BirA* for 18 hours were grown in media containing biotin (50 μM) for 6 h and double labelled with streptavidin-488 (green) and anti-myc (red). (B) Lists of identified interaction partners in KDELR1-BioID with a number of identified peptides (all and unique) with their sequence coverage. (C) Immunoblotting of KDELR1-BioID samples confirmed biotinylation of the candidate interaction partners identified in mass spectrometry. Anti-GM130 and anti-Golgin97 blots were included as negative controls here. (D - J) Confocal micrographs of HeLa cells expressing KDELR1-mCherry, showing that depletion of Giantin, GRASP55, Golgin160 and ARFGAP1/2/3 do not result in ER re-distribution of KDELR1-mCherry. (K) Western blots showing depletion of Giantin, GRASP55, Golgin160, ACBD3 and ArfGAP1/2/3, respectively, by RNA interference. Scale bars = 10 μm.