Back to Search
Start Over
Analysis of the optical response of a SARS-CoV-2-directed colorimetric immunosensor
- Source :
- AIP Advances, Vol 11, Iss 6, Pp 065319-065319-7 (2021)
- Publication Year :
- 2021
- Publisher :
- AIP Publishing LLC, 2021.
-
Abstract
- The optical response of different configurations of functionalized gold nanoparticles (f-AuNPs) and SARS-CoV-2 virions is simulated in order to explore the behavior of a colloidal solution containing 105–1013 virions/ml. The analysis herein reported is carried out for three concentration regimes: (i) low (≲108 virions/ml), (ii) intermediate (∼109–1010 virions/ml), and (iii) high (≳1011 virions/ml). Given the high binding effectiveness of f-AuNPs to virions, three different configurations are expected to arise: (i) virions completely surrounded by f-AuNPs, (ii) aggregates (dimers or trimers) of virions linked by f-AuNPs, and (iii) single f-AuNP surrounded by virions. It is demonstrated that 20 nm diameter gold nanoparticles functionalized against all three kinds of SARS-CoV-2 proteins (membrane, envelope, and spike) allow one to reach a limit of detection (LOD) of ∼106 virions/ml, whereas the use of only one kind of f-AuNP entails a ten-fold worsening of the LOD. It is also shown that the close proximity (∼5 nm) of the f-AuNP to the virions assumed throughout this analysis is essential to avoid the hook effect, thereby pointing out the importance of realizing an apt functionalization procedure that keeps thin the dielectric layer (e.g., proteins or aptamers) surrounding the gold nanoparticles.
- Subjects :
- 010302 applied physics
Detection limit
Chemistry
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)
Aptamer
viruses
Physics
QC1-999
General Physics and Astronomy
02 engineering and technology
biochemical phenomena, metabolism, and nutrition
021001 nanoscience & nanotechnology
Colloidal Solution
01 natural sciences
Membrane
Dielectric layer
Colloidal gold
0103 physical sciences
Biophysics
Surface modification
0210 nano-technology
Subjects
Details
- Language :
- English
- ISSN :
- 21583226
- Volume :
- 11
- Issue :
- 6
- Database :
- OpenAIRE
- Journal :
- AIP Advances
- Accession number :
- edsair.doi.dedup.....9104d3bb29affc14a627b1987ea0537d