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Loss of RASSF1A Expression in Colorectal Cancer and Its Association with K-ras Status
- Source :
- BioMed Research International, BioMed Research International, Vol 2013 (2013)
- Publication Year :
- 2013
- Publisher :
- Hindawi Publishing Corporation, 2013.
-
Abstract
- Background. The RAS-association domain family 1 A (RASSF1A) is a classical member of RAS effectors regulating cell proliferation and apoptosis. Loss of RASSF1A expression may shift the balance towards a growth-promoting effect without the necessity of activating K-ras mutations. Its potential association with K-ras mutations in colorectal cancer (CRC) is unclear. Methods. RASSF1A expression was examined in normal mucosa, adenoma, and tumor tissues of colon and rectum, respectively. We examined the association of RASSF1A expression, mutations of K-ras, and EGFR status in 76 primary CRCs. The relationship between clinicopathological characteristics and RASSF1A expression was also analyzed. Results. RASSF1A expression level decreased progressively in normal mucosa, adenoma and, tumor tissues, and the loss of RASSF1A expression occurred more frequently in tumor tissues. Of 76 primary CRCs, loss of RASSF1A expression and/or K-ras mutations were detected in 77% cases. Loss of RASSF1A expression was more frequent in K-ras wild-type than in mutation cases (63% versus 32%, P = 0.011). Conclusions. Our study indicates that loss of RASSF1A may be involved in pathogenesis of CRC, its expression was found predominantly in K-ras wild-type CRCs, suggesting that it may be another way of affecting RAS signaling, in addition to K-ras mutations.
- Subjects :
- Adenoma
Adult
Male
Pathology
medicine.medical_specialty
endocrine system
Article Subject
Colorectal cancer
DNA Mutational Analysis
Molecular Sequence Data
lcsh:Medicine
Rectum
Biology
medicine.disease_cause
General Biochemistry, Genetics and Molecular Biology
Pathogenesis
Proto-Oncogene Proteins p21(ras)
Proto-Oncogene Proteins
medicine
Humans
Intestinal Mucosa
Aged
Aged, 80 and over
Mutation
General Immunology and Microbiology
Base Sequence
Effector
Cell growth
Tumor Suppressor Proteins
lcsh:R
General Medicine
Middle Aged
medicine.disease
Immunohistochemistry
digestive system diseases
ErbB Receptors
medicine.anatomical_structure
Apoptosis
Cancer research
ras Proteins
Female
Colorectal Neoplasms
Research Article
Subjects
Details
- Language :
- English
- ISSN :
- 23146141 and 23146133
- Volume :
- 2013
- Database :
- OpenAIRE
- Journal :
- BioMed Research International
- Accession number :
- edsair.doi.dedup.....9114a8291c319311a8fffd04bf4e55cd