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Pathogen reduction with amotosalen/UVA reduces platelet refractoriness in a dog platelet transfusion model
- Source :
- Vox Sanguinis. 114:595-604
- Publication Year :
- 2019
- Publisher :
- Wiley, 2019.
-
Abstract
- Background and objectives Pathogen reduction of donor platelets with amotosalen/UVA has been shown to effectively inactivate pathogens and also contaminating white blood cells (WBCs). We wanted to determine whether WBC inactivation could also decrease alloimmune refractoriness to donor platelets. Materials and methods Platelets were prepared from a donor dog's whole blood, and the platelets were either transfused without modification [standard (STD) platelets] or treated with amotosalen/UVA under conditions modelling the amotosalen/UVA Blood System for human platelets (APR) using either 4 or 3 J/cm2 of UVA exposure. Platelets were transfused weekly from a single donor dog for 8 weeks or until the recipient dog became refractory to their donor's platelets. Antibody samples were drawn weekly and tested against the donor dog's platelets and WBCs (CD8 and B cells). Results Only 1/7 (14%) dogs that received STD platelets accepted 8 weeks of donor transfusions. Following APR 4 J/cm2 donor transfusions, 3/9 (33%) recipients accepted their donor's transfusions, but only one recipient remained antibody negative. Following APR 3 J/cm2 donor transfusions, the same dose as used for human platelet transfusions, 7/10 (70%) recipients accepted their donor's transfusions, but only two remained antibody negative. Conclusion As a very high percentage of recipient dogs (70%) accepted APR 3 J/cm2 donor transfusions, these data suggest that preventing alloimmune platelet refractoriness may be another benefit of pathogen reduction using amotosalen/UVA.
- Subjects :
- Male
Amotosalen
Ultraviolet Rays
Pathogen reduction
Blood Donors
Platelet Transfusion
030204 cardiovascular system & hematology
03 medical and health sciences
Dogs
0302 clinical medicine
Refractory
Furocoumarins
Animals
Medicine
Blood Transfusion
Platelet
Whole blood
biology
business.industry
Hematology
General Medicine
Platelet transfusion
Models, Animal
Immunology
biology.protein
Female
Antibody
business
CD8
030215 immunology
Subjects
Details
- ISSN :
- 14230410 and 00429007
- Volume :
- 114
- Database :
- OpenAIRE
- Journal :
- Vox Sanguinis
- Accession number :
- edsair.doi.dedup.....914e384bb78a56c1d7c300f0799edc61