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MicroRNA-200b Is Overexpressed in Endometrial Adenocarcinomas and EnhancesMMP2Activity by DownregulatingTIMP2in Human Endometrial Cancer Cell Line HEC-1A Cells
- Source :
- Nucleic Acid Therapeutics. 23:29-34
- Publication Year :
- 2013
- Publisher :
- Mary Ann Liebert Inc, 2013.
-
Abstract
- MicroRNAs (miRNAs) play important roles in tumorigenesis and metastasis. In this study, we investigated miR-200b expression in endometrial adenocarcinomas and normal adjacent tissues and found that miR-200b is more highly expressed in cancer tissues than in normal adjacent tissues. A novel target of miR-200b, tissue inhibitor of metalloproteinase 2 (TIMP2), was predicted using a bioinformatics approach and was confirmed in human endometrial cancer cell line HEC-1A cells by luciferase assay, quantitative real-time polymerase chain reaction, western blotting, and enzyme-linked immunosorbent assay. We found that miR-200b repressed TIMP2 expression at both the messenger RNA and protein levels, although a family member, miR-200a, had no such effect. Using reverse gelatin zymography, we showed that miR-200b enhances matrix metallopeptidase 2 (MMP2) activity by downregulating TIMP2 expression in HEC-1A cells. These data suggest that miR-200b may play an important role in the metastasis of endometrial adenocarcinomas.
- Subjects :
- MMP2
Down-Regulation
Adenocarcinoma
Biology
medicine.disease_cause
Biochemistry
Metastasis
Cell Line, Tumor
Drug Discovery
microRNA
Genetics
medicine
Humans
RNA, Messenger
RNA, Neoplasm
3' Untranslated Regions
Molecular Biology
Tissue Inhibitor of Metalloproteinase-2
Base Sequence
Endometrial cancer
Cancer
Original Articles
Tissue inhibitor of metalloproteinase
medicine.disease
Molecular biology
Endometrial Neoplasms
MicroRNAs
Matrix Metalloproteinase 2
Molecular Medicine
Female
Carcinogenesis
Subjects
Details
- ISSN :
- 21593345 and 21593337
- Volume :
- 23
- Database :
- OpenAIRE
- Journal :
- Nucleic Acid Therapeutics
- Accession number :
- edsair.doi.dedup.....91636642a3b3cd74ef047bd8e4ee5fc7