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Characterization of two galactosidases extracted from wheat germ with a hydroalcoholic solvent
- Source :
- Biochimie. 74(1)
- Publication Year :
- 1992
-
Abstract
- Alpha- and beta-D-galactosidases were characterized from a hydroalcoholic extract of wheat germ (Triticum vulgare). Kinetic constants (Vmax and KM) and the optimal pHs for the hydrolysis of p-nitrophenyl galactopyranosides by both enzymes were determined. These enzymes presented a high stability in hydroalcoholic medium and were inhibited by iodoacetamide and sodium p-hydroxy-mercuribenzoate.
- Subjects :
- Sodium
chemistry.chemical_element
Acetates
Biochemistry
Hydrolysis
chemistry.chemical_compound
Glycoside hydrolase
Triticum
Acetic Acid
chemistry.chemical_classification
Chromatography
Galactosidases
biology
Ethanol
Temperature
food and beverages
General Medicine
Hydrogen-Ion Concentration
Solvent
Enzyme
chemistry
Enzyme inhibitor
biology.protein
Iodoacetamide
Subjects
Details
- ISSN :
- 03009084
- Volume :
- 74
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Biochimie
- Accession number :
- edsair.doi.dedup.....91819974fc633d26c2444e0e3e305b5d