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Metabolomics of Fuzi-Gancao in CCl4 induced acute liver injury and its regulatory effect on bile acid profile in rats
- Source :
- World Journal of Gastroenterology
- Publication Year :
- 2021
- Publisher :
- Baishideng Publishing Group Inc, 2021.
-
Abstract
- BACKGROUND Fuzi (Radix aconiti lateralis)-Gancao (Radix glycyrrhizae) is one of the most classical drug pairs of traditional Chinese medicine. In clinical practice, decoctions containing Fuzi-Gancao (F-G) are often used in the treatment of liver diseases such as hepatitis and liver failure. AIM To investigate the metabolomics of F-G in CCl4 induced acute liver injury in rats and its regulatory effect on the bile acid profile. METHODS The pharmacodynamic effect of F-G on CCl4 induced acute liver injury in rats was evaluated, and an ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method for the simultaneous determination of 92 metabolites from multiple pathways was established to explore the protective metabolic mechanism of F-G in serum on the liver. RESULTS Twenty-four differential metabolites were identified in serum samples. The primary bile acid biosynthetic metabolic pathway was the major common pathway in the model group and F-G group. Subsequently, a UPLC-MS/MS method for simultaneous determination of 11 bile acids, including cholic acid, ursodeoxycholic acid, glycochenodeoxycholic acid, glycochenodeoxycholic acid, taurocholic acid, glycocholic acid, chenodeoxycholic acid, deoxycholic acid, taurochenodeoxycholic acid, taurocholic acid, and glycinic acid, was established to analyze the regulatory mechanism of F-G in serum. F-G decreased the contents of these 11 bile acids in serum in a dose-dependent manner compared with those in the model control group. CONCLUSION F-G could protect hepatocytes by promoting the binding of free bile acids to glycine and taurine, and reducing the accumulation of free bile acids in the liver. F-G could also regulate the compensatory degree of taurine, decreasing the content of taurine-conjugated bile acids to protect hepatocytes.
- Subjects :
- medicine.drug_class
CCL4
Bile acid
Pharmacology
Liver injury
digestive system
Bile Acids and Salts
Metabolomics
Tandem Mass Spectrometry
parasitic diseases
medicine
Metabolites
Glycyrrhiza
Animals
Acute liver injury
business.industry
Gastroenterology
General Medicine
Basic Study
medicine.disease
digestive system diseases
Rats
Fuzi-Gancao
Liver
Radix glycyrrhizae
Radix aconiti lateralis
Diterpenes
business
Chromatography, Liquid
Drugs, Chinese Herbal
Subjects
Details
- Language :
- English
- ISSN :
- 22192840 and 10079327
- Volume :
- 27
- Issue :
- 40
- Database :
- OpenAIRE
- Journal :
- World Journal of Gastroenterology
- Accession number :
- edsair.doi.dedup.....91820a24b1cacb119018f8f68b0bf389