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CHROMATO-MASS SPECTROMETRIC ANALYSIS OF URINE PROTEINS ASSOCIATED WITH THE FUNCTIONS OF TOLL-RECEPTORS IN A HEALTHY PERSON UNDER CONDITIONS OF 17-DAY ISOLATION
- Source :
- Russian Clinical Laboratory Diagnostics. 65:469-473
- Publication Year :
- 2020
- Publisher :
- EKOlab, 2020.
-
Abstract
- Under controlled conditions of 17-day isolation (Sirius-17 experiment), the protein composition of urine was studied in 6 healthy test volunteers-3 women and 3 men. Collection of samples in the form of a second freely separated morning urine fraction was carried out in the background (seven days before the experiment), as well as 1 day after the end of exposure. Chromatographic-mass-spectrometric semi-quantitative analysis of the protein composition of samples was performed on a system consisting of an Agilent 1100 chromatograph and an LTQ-FT Ultra hybrid mass spectrometer using bioinformatics resources UniProtKB, GeneOntology. An asymptomatic change in the immune defense system of kidney tissue after isolation in a closed hermetic object is associated with a change in the content of 7 proteins that provide functional activity of the TLR tubules of the kidneys - FcRIII, MUC1, Galectin-3, Ficolin-2, APOA1, FLNA, FCGR3A and Clusterin. These proteins are found to be useful biomarkers in the study of physiology and kidney diseases. They can be attributed to candidates for protein markers of the initial stages of impaired recognition by the epithelium of renal tubules of bacteria with known pathogenic potential.
- Subjects :
- Male
0301 basic medicine
Proteome
Urine
Urinalysis
Mass Spectrometry
03 medical and health sciences
0302 clinical medicine
medicine
Humans
FLNA
Receptor
MUC1
Chromatography
Kidney
Clusterin
biology
Chemistry
Receptors, IgG
Biochemistry (medical)
FCGR3A
General Medicine
Epithelium
Medical Laboratory Technology
030104 developmental biology
medicine.anatomical_structure
Biochemistry
biology.protein
Biomarkers
030217 neurology & neurosurgery
Subjects
Details
- ISSN :
- 24121320 and 08692084
- Volume :
- 65
- Database :
- OpenAIRE
- Journal :
- Russian Clinical Laboratory Diagnostics
- Accession number :
- edsair.doi.dedup.....919a76aa10c283345b58deb27fb46e99
- Full Text :
- https://doi.org/10.18821/0869-2084-2020-65-8-469-473