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A Faster, Novel Technique to Detect COVID-19 Neutralizing Antibodies

Authors :
Wei-Huai Chiu
Wei-Yi Kong
Yu-Jang Su
Jyun-Wei Wen
Ciao-Ming Tsai
Chitsung Hong
Pang-Yen Chen
Cheng-Hao Ko
Source :
Medical Science Monitor. 28
Publication Year :
2022
Publisher :
International Scientific Information, Inc., 2022.

Abstract

BACKGROUND The COVID-19 pandemic has spread globally in a short period of time. It is known that antibody (nAb) level can effectively predict vaccine efficacy, which leads to the exploration of vaccine trials for efficacy assessment. Thus, the current study aimed to develop a platform to quantify nAb levels faster, at lower cost, and with better efficiency. MATERIAL AND METHODS A total of 69 sera samples were collected for the research, 28 of which were from unvaccinated participants. The other 27 samples and the remaining 14 samples were from the participants who had received the first and second dose, respectively, of AZ vaccine 1 month before. With cPass assays (Genscript cPass nAb ELISA assay) used as a criterion standard and lateral flow immunoassay kit (Healgen Scientific - LFIA test kit) coupled with a spectrometer (LFIA+S) for checking each specimen, we aimed to detect the presence of neutralizing antibodies in sera and to confirm the relationship between the inhibition rate from cPass assays and the nAb index from the LFIA+S. RESULTS Data analysis of the research were taken from the certified ELISA and LFIA+S, which indicated a high consistency (Pearson's r =0.864; ICC=0.90138) between the 2 methods. CONCLUSIONS The dataset demonstrated that LFIA+S was affordable, had a strong correlation with results of the cPass nAbs detection kit, and has potential clinical applications, with an exclusive feature that allows non-experts to use it with ease. It is believed that the proposed platform can be promoted in the near future.

Details

ISSN :
16433750
Volume :
28
Database :
OpenAIRE
Journal :
Medical Science Monitor
Accession number :
edsair.doi.dedup.....91c15f0e82db03e3dc7ca8940d0594a2