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Hydrothermal Reduction of Polyethylenimine and Polyethylene Glycol Dual-Functionalized Nanographene Oxide for High-Efficiency Gene Delivery
- Source :
- ACS Applied Materials & Interfaces. 8:31311-31320
- Publication Year :
- 2016
- Publisher :
- American Chemical Society (ACS), 2016.
-
Abstract
- In this study, a physiologically stable dual-polymer-functionalized reduced nanographene oxide (nrGO) conjugate (PEG–nrGO–PEI, RGPP) with high efficiency of gene delivery is successfully synthesized through mixing PEGylated nanographene oxide (PEG–nGO, GP) and polyethylenimine (PEI, 25 kDa) solution under 80 °C for 2 h. This hydrothermal reduction of GP during PEIylation promotes the nucleophilic reaction between the amino moieties of PEI and the epoxy groups (or carboxylic groups) in GP and then forms C–NH– groups (or NH–CO groups) to covalently connect PEI and GP, which makes the RGPP nanocomposite more stable in physiological environments and has superior gene transfection efficiency compared with the nonhydrothermally reduced PEG–nGO/PEI conjugate (GPP) obtained by mixing GP and PEI under 20 °C for 2 h. Moreover, 808 nm laser irradiation (2 W/cm2) for 25 min increases ∼1.5-fold of gene transfection efficiency for RGPP but does not increase the gene transfection efficiency of GPP. Finally, RGPP is also...
- Subjects :
- Materials science
Oxide
macromolecular substances
02 engineering and technology
Polyethylene glycol
Gene delivery
Transfection
010402 general chemistry
01 natural sciences
Polyethylene Glycols
chemistry.chemical_compound
PEG ratio
Polyethyleneimine
Organic chemistry
General Materials Science
Polyethylenimine
Nanocomposite
Gene Transfer Techniques
technology, industry, and agriculture
Oxides
021001 nanoscience & nanotechnology
Nanostructures
0104 chemical sciences
chemistry
Covalent bond
Graphite
0210 nano-technology
Conjugate
Nuclear chemistry
Subjects
Details
- ISSN :
- 19448252 and 19448244
- Volume :
- 8
- Database :
- OpenAIRE
- Journal :
- ACS Applied Materials & Interfaces
- Accession number :
- edsair.doi.dedup.....91ce4aecb49adc3d3ad83e9e5de7fcda