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Molecular characterization of Bathymodiolus mussels and gill symbionts associated with chemosynthetic habitats from the U.S. Atlantic margin
- Source :
- PLoS ONE, Vol 14, Iss 3, p e0211616 (2019), PLoS ONE
- Publication Year :
- 2019
- Publisher :
- Public Library of Science (PLoS), 2019.
-
Abstract
- Mussels of the genus Bathymodiolus are among the most widespread colonizers of hydrothermal vent and cold seep environments, sustained by endosymbiosis with chemosynthetic bacteria. Presumed species of Bathymodiolus are abundant at newly discovered cold seeps on the Mid-Atlantic continental slope, however morphological taxonomy is challenging, and their phylogenetic affinities remain unestablished. Here we used mitochondrial sequence to classify species found at three seep sites (Baltimore Canyon seep (BCS; ~400m); Norfolk Canyon seep (NCS; ~1520m); and Chincoteague Island seep (CTS; ~1000m)). Mitochondrial COI (N = 162) and ND4 (N = 39) data suggest that Bathymodiolus childressi predominates at these sites, although single B. mauritanicus and B. heckerae individuals were detected. As previous work had suggested that methanotrophic and thiotrophic interactions can both occur at a site, and within an individual mussel, we investigated the symbiont communities in gill tissues of a subset of mussels from BCS and NCS. We constructed metabarcode libraries with four different primer sets spanning the 16S gene. A methanotrophic phylotype dominated all gill microbial samples from BCS, but sulfur-oxidizing Campylobacterota were represented by a notable minority of sequences from NCS. The methanotroph phylotype shared a clade with globally distributed Bathymodiolus spp. symbionts from methane seeps and hydrothermal vents. Two distinct Campylobacterota phylotypes were prevalent in NCS samples, one of which shares a clade with Campylobacterota associated with B. childressi from the Gulf of Mexico and the other with Campylobacterota associated with other deep-sea fauna. Variation in chemosynthetic symbiont communities among sites and individuals has important ecological and geochemical implications and suggests shifting reliance on methanotrophy. Continued characterization of symbionts from cold seeps will provide a greater understanding of the ecology of these unique environments as well and their geochemical footprint in elemental cycling and energy flux.
- Subjects :
- Gills
0106 biological sciences
0301 basic medicine
Heredity
Bathymodiolus
Pathology and Laboratory Medicine
01 natural sciences
Database and Informatics Methods
RNA, Ribosomal, 16S
Medicine and Health Sciences
Mussels
Atlantic Ocean
Phylogeny
Data Management
Chemosynthesis
Phylotype
Multidisciplinary
Ecology
Microbiota
Eukaryota
Phylogenetic Analysis
Biodiversity
Genomics
Bacterial Pathogens
Phylogenetics
Genetic Mapping
Medical Microbiology
Medicine
Pathogens
Sequence Analysis
Research Article
Hydrothermal vent
Bivalves
Computer and Information Sciences
Bioinformatics
Science
Sequence Databases
Biology
Research and Analysis Methods
DNA, Mitochondrial
Microbiology
010603 evolutionary biology
03 medical and health sciences
Genetics
Animals
Evolutionary Systematics
Symbiosis
Microbial Pathogens
Taxonomy
Evolutionary Biology
Sequence Assembly Tools
Bacteria
Organisms
Biology and Life Sciences
Computational Biology
Campylobacter
Molluscs
Mussel
Genome Analysis
biology.organism_classification
Invertebrates
Cold seep
Petroleum seep
Biological Databases
030104 developmental biology
Haplotypes
Mytilidae
Sequence Alignment
Subjects
Details
- Language :
- English
- ISSN :
- 19326203
- Volume :
- 14
- Issue :
- 3
- Database :
- OpenAIRE
- Journal :
- PLoS ONE
- Accession number :
- edsair.doi.dedup.....92083554127bcede3d88e191c94d8b96