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The effects of intra-dorsal hippocampus infusion of pregnenolone sulfate on memory function and hippocampal BDNF mRNA expression of biliary cirrhosis-induced memory impairment in rats
- Source :
- Neuroscience. 306:1-9
- Publication Year :
- 2015
- Publisher :
- Elsevier BV, 2015.
-
Abstract
- Learning and memory impairment is one of the most challenging complications of cirrhosis and present treatments are unsatisfactory. The exact mechanism of cirrhosis cognitive dysfunction is unknown. Pregnenolone sulfate (PREGS) is an excitatory neurosteroid that acts as a N-methyl-D-aspartate (NMDA) receptor agonist and GABAA receptor antagonist. In this study we evaluated the effect of intra CA1 infusion of PREGS on cirrhotic rats' memory function using the Y-maze test. Hippocampal brain-derived neurotrophic factor (BDNF) mRNA expression was also evaluated. Three weeks after bile duct ligation (BDL) surgery, rats were under stereotaxic surgery for insertion of two guide cannulas in the CA1 region of the hippocampus. After 1-week of recovery, PREGS was administered through CA1 cannulas in cirrhotic rats, while control or sham groups received vehicle. For evaluation of NMDA receptor role in memory-enhancing effects of PREGS, DL-2-Amino-5-phosphonopentanoic acid (AP5) which is a potent and competitive antagonist of NMDA receptor, co-administered with PREGS and for assessment of hippocampal BDNF mRNA expression, quantitative Real-time reverse transcriptase-PCR (RT-PCR) was used. Results showed that 28 days after BDL, cirrhotic animals' memory significantly decreased in comparison with control and sham groups, while PREGS infusion could restore memory impairment (P
- Subjects :
- Male
Agonist
medicine.medical_specialty
Neuroactive steroid
medicine.drug_class
Hippocampus
Receptors, N-Methyl-D-Aspartate
chemistry.chemical_compound
Memory
Internal medicine
medicine
Animals
GABA-A Receptor Antagonists
RNA, Messenger
Rats, Wistar
CA1 Region, Hippocampal
Brain-derived neurotrophic factor
Memory Disorders
Liver Cirrhosis, Biliary
business.industry
Brain-Derived Neurotrophic Factor
General Neuroscience
Long-term potentiation
Rats
Disease Models, Animal
Endocrinology
2-Amino-5-phosphonovalerate
nervous system
chemistry
Pregnenolone
NMDA receptor
Bile Ducts
Pregnenolone sulfate
business
medicine.drug
Subjects
Details
- ISSN :
- 03064522
- Volume :
- 306
- Database :
- OpenAIRE
- Journal :
- Neuroscience
- Accession number :
- edsair.doi.dedup.....92142ffc0f333c2f68b0df4cce46ed54