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Epithelial eotaxin-2 and eotaxin-3 expression: relation to asthma severity, luminal eosinophilia and age at onset
- Source :
- Thorax. 67:1061-1066
- Publication Year :
- 2012
- Publisher :
- BMJ, 2012.
-
Abstract
- Background Eosinophilic inflammation is implicated in asthma. Eotaxin 1–3 regulate eosinophil trafficking into the airways along with other chemotactic factors. However, the epithelial and bronchoalveolar lavage (BAL) cell expression of these chemokines in relation to asthma severity and eosinophilic phenotypes has not been addressed. Objective To measure the expression of the three eotaxin isoforms in bronchoscopically obtained samples and compare them with clinically relevant parameters between normal subjects and patients with asthma. Methods Normal subjects and patients with asthma of varying severity recruited through the Severe Asthma Research Program underwent clinical assessment and bronchoscopy with airway brushing and BAL. Eotaxin 1–3 mRNA/protein were measured in epithelial and BAL cells and compared with asthma severity, control and eosinophilic inflammation. Results Eotaxin-2 and eotaxin-3 mRNA and eotaxin-2 protein were increased in airway epithelial brushings from patients with asthma and were highest in cases of severe asthma (p values 0.0155, 0.0033 and 0.0006, respectively), with eotaxin-2 protein increased with age at onset. BAL cells normally expressed high levels of eotaxin-2 mRNA/protein but BAL fluid levels of eotaxin-2 were lowest in severe asthma. Epithelial eotaxin-2 and eotaxin-3 mRNA/protein was associated with sputum eosinophilia, lower forced expiratory volume in 1 s and more asthma exacerbations. Airway epithelial cell eotaxin-2 protein differed by asthma severity only in those with late onset disease, and tended to be highest in those with late onset eosinophilic asthma. Conclusions Epithelial eotaxin-2 and 3 are increased in asthma and severe asthma. Their expression may contribute to luminal migration of eosinophils, especially in later onset disease, asthma control and severity.
- Subjects :
- Adult
Chemokine CCL11
Male
Pulmonary and Respiratory Medicine
Eotaxin
Enzyme-Linked Immunosorbent Assay
Inflammation
Late onset
Real-Time Polymerase Chain Reaction
Bronchoalveolar Lavage
Article
Statistics, Nonparametric
Adrenal Cortex Hormones
immune system diseases
Bronchoscopy
Eosinophilia
Eosinophilic
medicine
Humans
Age of Onset
Asthma
medicine.diagnostic_test
Chemokine CCL26
business.industry
Chemokine CCL24
Epithelial Cells
respiratory system
Eosinophil
medicine.disease
respiratory tract diseases
medicine.anatomical_structure
Bronchoalveolar lavage
Chemokines, CC
Immunology
Female
medicine.symptom
business
Subjects
Details
- ISSN :
- 14683296 and 00406376
- Volume :
- 67
- Database :
- OpenAIRE
- Journal :
- Thorax
- Accession number :
- edsair.doi.dedup.....921fc25446ad53a8af3d82bb97ce3e55