Back to Search
Start Over
Saponins from Panax japonicus ameliorate age-related renal fibrosis by inhibition of inflammation mediated by NF-κB and TGF-β1/Smad signaling and suppression of oxidative stress via activation of Nrf2-ARE signaling
- Source :
- Journal of Ginseng Research, Journal of Ginseng Research, Vol 45, Iss 3, Pp 408-419 (2021)
- Publication Year :
- 2021
- Publisher :
- Elsevier BV, 2021.
-
Abstract
- Background: The decreased renal function is known to be associated with biological aging, of which the main pathological features are chronic inflammation and renal interstitial fibrosis. In previous studies, we reported that total saponins from Panax japonicus (SPJs) can availably protect acute myocardial ischemia. We proposed that SPJs might have similar protective effects for aging-associated renal interstitial fibrosis. Thus, in the present study, we evaluated the overall effect of SPJs on renal fibrosis. Methods: Sprague-Dawley (SD) aging rats were given SPJs by gavage beginning from 18 months old, at 10 mg/kg/d and 60 mg/kg/d, up to 24 months old. After the experiment, changes in morphology, function and fibrosis of their kidneys were detected. The levels of serum uric acid (UA), β2-microglobulin (β2-MG) and cystatin C (Cys C) were assayed with ELISA kits. The levels of extracellular matrix (ECM), matrix metalloproteinases (MMPs), tissue inhibitors of metalloproteinases (TIMPs), inflammatory factors and changes of oxidative stress parameters were examined. Results: After SPJs treatment, SD rats showed significantly histopathological changes in kidneys accompanied by decreased renal fibrosis and increased renal function; As compared with those in 3-month group, the levels of serum UA, Cys C and β2-MG in 24-month group were significantly increased (p
- Subjects :
- 0301 basic medicine
Aging
Cys C, cystatin C
SPJ-L, low-dose of SPJ
TIMPs, tissue inhibitors of metalloproteinases
SMAD
Matrix metalloproteinase
medicine.disease_cause
TNF-α, tumor necrosis factor-α
Nrf2, nuclear factor erythroid 2-related factor 2
α-SMA, α-smooth muscle aorta
0302 clinical medicine
Renal fibrosis
Fibrosis
HO-1, human heme oxygenase 1
TGF-β1, tumor growth factor-β1
IL-6, interleukin-6
IκB, inhibitor of NF-κB
biology
NQO1, recombinant NADH dehydrogenase quinone 1
Total saponins of panax japonicus
MCP-1, monocyte chemoattractant protein-1
ECM, extracellular matrix
UA, uric acid
SPJ-H, high-dose of SPJ
030220 oncology & carcinogenesis
medicine.symptom
Research Article
Biotechnology
medicine.medical_specialty
SPJs, saponins from panax japonicus
ARE, antioxidant response element
Inflammation
Biochemistry, Genetics and Molecular Biology (miscellaneous)
03 medical and health sciences
Internal medicine
medicine
PJ, Panax japonicas
COX2, cyclooxygenase-2
LPO, lipid peroxides
business.industry
TGF-β1/Smad
Botany
SD, Sprague-Dawley
medicine.disease
NF-κB, nuclear factor kappa-B
030104 developmental biology
Endocrinology
Complementary and alternative medicine
Cystatin C
QK1-989
Nrf2-ARE signaling pathways
biology.protein
MMPs, matrix metalloproteinases
β2-MG, β2-microglobulin
business
Oxidative stress
Transforming growth factor
Subjects
Details
- ISSN :
- 12268453
- Volume :
- 45
- Database :
- OpenAIRE
- Journal :
- Journal of Ginseng Research
- Accession number :
- edsair.doi.dedup.....92217cdd10e9c934df2aec2229c0abbf