Supplementary Figure 4 from COX-2–Independent Effects of Celecoxib Sensitize Lymphoma B Cells to TRAIL-Mediated Apoptosis

PDF file - 75K,(A) Expression of the 4 EP receptors (PGE2 receptors) in BL2 and SUDHL4 was evaluated by western blotting. (B) and (C) BL2 and SUDHL4 were cultured with X-VIVO medium and stimulated with PGE2 for 24h. (B) B cell proliferation was evaluated with tritiated thymidine (3H-TdR) incorporation. Results from one of three representative experiments. Mean plus-minus SD. (C) After PGE2 stimulation for 1h, BL2 and SUDHL4 were treated or not with 100ng/ml TRAIL for 24h. Apoptotic B cells were analysed by flow cytometry using CD19pos, CD20pos and active caspase-3 staining.