Back to Search
Start Over
Antioxidant, Xanthine Oxidase, α-Amylase and α-Glucosidase Inhibitory Activities of Bioactive Compounds from Rumex crispus L. Root
- Source :
- Molecules, Volume 24, Issue 21, Molecules, Vol 24, Iss 21, p 3899 (2019)
- Publication Year :
- 2019
- Publisher :
- Multidisciplinary Digital Publishing Institute, 2019.
-
Abstract
- The root of Rumex crispus L. has been shown to possess anti-gout and anti-diabetic properties, but the compounds responsible for these pharmaceutical effects have not yet been reported. In this study, we aimed to isolate and purify active components from the root of R. crispus, and to evaluate their anti-radical, anti-gout and anti-diabetic capacities. From the ethyl acetate (EtOAc) extract, two compounds, chrysophanol (1) and physcion (2), were isolated by column chromatography with an elution of hexane and EtOAc at a 9:1 ratio. Their structures were identified by spectrometric techniques including gas chromatography-mass spectrometry (GC-MS), electrospray ionization-mass spectrometry (ESI-MS), X-ray diffraction analyses and nuclear magnetic resonance (NMR). The results of bioassays indicated that (1) showed stronger activities than (2). For antioxidant activity, (1) and (2) exhibited remarkable DPPH radical scavenging capacity (IC50 = 9.8 and 12.1 &micro<br />g/mL), which was about two times stronger than BHT (IC50 = 19.4 &micro<br />g/mL). The anti-gout property of (1) and (2) were comparable to the positive control allopurinol, these compounds exerted strong inhibition against the activity of xanthine oxidase (IC50 = 36.4 and 45.0 &micro<br />g/mL, respectively). In the anti-diabetic assay, (1) and (2) displayed considerable inhibitory ability on &alpha<br />glucosidase, their IC50 values (IC50 = 20.1 and 18.9 &micro<br />g/mL, respectively) were higher than that of standard acarbose (IC50 = 143.4 &micro<br />g/mL). Findings of this study highlight that (1) and (2) may be promising agents to treat gout and diabetes, which may greatly contribute to the medicinal properties of Rumex crispus root.
- Subjects :
- Antioxidant
DPPH
medicine.medical_treatment
Phytochemicals
Ethyl acetate
Pharmaceutical Science
Plant Roots
01 natural sciences
Antioxidants
Analytical Chemistry
chemistry.chemical_compound
Drug Discovery
Amylase
physcion
0303 health sciences
biology
Bioactive compound
anti-diabetic
Chemistry (miscellaneous)
Molecular Medicine
Xanthine Oxidase
Article
lcsh:QD241-441
03 medical and health sciences
Column chromatography
lcsh:Organic chemistry
medicine
Glycoside Hydrolase Inhibitors
Carbon-13 Magnetic Resonance Spectroscopy
Physical and Theoretical Chemistry
Rumex
Xanthine oxidase
bioactive compound
030304 developmental biology
Rumex crispus
Chromatography
Plant Extracts
010405 organic chemistry
anti-gout
Organic Chemistry
chrysophanol
alpha-Glucosidases
anti-radical
biology.organism_classification
0104 chemical sciences
chemistry
biology.protein
alpha-Amylases
Subjects
Details
- Language :
- English
- ISSN :
- 14203049
- Database :
- OpenAIRE
- Journal :
- Molecules
- Accession number :
- edsair.doi.dedup.....92a02b0d6871c451e5caaa9381c250fb
- Full Text :
- https://doi.org/10.3390/molecules24213899