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Abnormal Epithelial Cell Polarity and Ectopic Epidermal Growth Factor Receptor (EGFR) Expression Induced in Emx2 KO Embryonic Gonads
- Source :
- Endocrinology. 151:5893-5904
- Publication Year :
- 2010
- Publisher :
- The Endocrine Society, 2010.
-
Abstract
- The gonadal primordium first emerges as a thickening of the embryonic coelomic epithelium, which has been thought to migrate mediodorsally to form the primitive gonad. However, the early gonadal development remains poorly understood. Mice lacking the paired-like homeobox gene Emx2 display gonadal dysgenesis. Interestingly, the knockout (KO) embryonic gonads develop an unusual surface accompanied by aberrant tight junction assembly. Morphological and in vitro cell fate mapping studies showed an apparent decrease in the number of the gonadal epithelial cells migrated to mesenchymal compartment in the KO, suggesting that polarized cell division and subsequent cell migration are affected. Microarray analyses of the epithelial cells revealed significant up-regulation of Egfr in the KO, indicating that Emx2 suppresses Egfr gene expression. This genetic correlation between the two genes was reproduced with cultured M15 cells derived from mesonephric epithelial cells. Epidermal growth factor receptor signaling was recently shown to regulate tight junction assembly through sarcoma viral oncogene homolog tyrosine phosphorylation. We show through Emx2 KO analyses that sarcoma viral oncogene homolog tyrosine phosphorylation, epidermal growth factor receptor tyrosine phosphorylation, and Egfr expression are up-regulated in the embryonic gonad. Our results strongly suggest that Emx2 is required for regulation of tight junction assembly and allowing migration of the gonadal epithelia to the mesenchyme, which are possibly mediated by suppression of Egfr expression.
- Subjects :
- endocrine system
medicine.medical_specialty
Mesenchyme
Protein Array Analysis
Apoptosis
Cell fate determination
Tight Junctions
Mice
chemistry.chemical_compound
Endocrinology
Internal medicine
medicine
Animals
Epidermal growth factor receptor
Gonads
Cell Proliferation
Homeodomain Proteins
Mice, Knockout
Tight junction
biology
Gene Expression Profiling
Gene Expression Regulation, Developmental
Epithelial Cells
Tyrosine phosphorylation
Cell migration
Coelomic epithelium
ErbB Receptors
medicine.anatomical_structure
chemistry
biology.protein
Abnormal Epithelial Cell
Transcription Factors
Subjects
Details
- ISSN :
- 19457170 and 00137227
- Volume :
- 151
- Database :
- OpenAIRE
- Journal :
- Endocrinology
- Accession number :
- edsair.doi.dedup.....92de0a799f66345faf24440f39b11ac5
- Full Text :
- https://doi.org/10.1210/en.2010-0915