Back to Search
Start Over
HPV Population Profiling in Healthy Men by Next-Generation Deep Sequencing Coupled with HPV-QUEST
- Source :
- Viruses, Vol 8, Iss 2, p 28 (2016), Viruses, Viruses; Volume 8; Issue 2; Pages: 28
- Publication Year :
- 2016
- Publisher :
- MDPI AG, 2016.
-
Abstract
- Multiple-type human papillomaviruses (HPV) infection presents a greater risk for persistence in asymptomatic individuals and may accelerate cancer development. To extend the scope of HPV types defined by probe-based assays, multiplexing deep sequencing of HPV L1, coupled with an HPV-QUEST genotyping server and a bioinformatic pipeline, was established and applied to survey the diversity of HPV genotypes among a subset of healthy men from the HPV in Men (HIM) Multinational Study. Twenty-one HPV genotypes (12 high-risk and 9 low-risk) were detected in the genital area from 18 asymptomatic individuals. A single HPV type, either HPV16, HPV6b or HPV83, was detected in 7 individuals, while coinfection by 2 to 5 high-risk and/or low-risk genotypes was identified in the other 11 participants. In two individuals studied for over one year, HPV16 persisted, while fluctuations of coinfecting genotypes occurred. HPV L1 regions were generally identical between query and reference sequences, although nonsynonymous and synonymous nucleotide polymorphisms of HPV16, 18, 31, 35h, 59, 70, 73, cand85, 6b, 62, 81, 83, cand89 or JEB2 L1 genotypes, mostly unidentified by linear array, were evident. Deep sequencing coupled with HPV-QUEST provides efficient and unambiguous classification of HPV genotypes in multiple-type HPV infection in host ecosystems.
- Subjects :
- Male
0301 basic medicine
Nonsynonymous substitution
asymptomatic men
lcsh:QR1-502
lcsh:Microbiology
0302 clinical medicine
HPV-QUEST
Genotype
Papillomaviridae
Phylogeny
education.field_of_study
HPV infection
High-Throughput Nucleotide Sequencing
virus diseases
Papillomavirus Episteme
female genital diseases and pregnancy complications
3. Good health
Infectious Diseases
030220 oncology & carcinogenesis
Coinfection
medicine.symptom
Adult
HPV
Molecular Sequence Data
Population
Asymptomatic
Article
Deep sequencing
Linear Array
multiple-type HPV infection
deep sequencing
Young Adult
03 medical and health sciences
Virology
medicine
Humans
education
Genotyping
Internet
Base Sequence
business.industry
Papillomavirus Infections
Computational Biology
medicine.disease
030104 developmental biology
business
Subjects
Details
- Language :
- English
- ISSN :
- 19994915
- Volume :
- 8
- Issue :
- 2
- Database :
- OpenAIRE
- Journal :
- Viruses
- Accession number :
- edsair.doi.dedup.....934eb514954e75e436aae29f44d39f25