Additional file 7 of SARS-CoV-2 detection status associates with bacterial community composition in patients and the hospital environment

Additional file 6: Figure S6. Floor sample SARS-CoV-2 status is associated with higher biomass and with significantly different bacterial community composition. Two independent metrics were used to assess biomass; 16S rRNA gene amplicon sequencing read count, which because of our equal volume sequencing library pooling approach correlates with total bacterial load [27, 74], and the Ct value from the CDC’s human RNAse P RT-qPCR target, which correlates with human biomass. (A) Abundance of 16S rRNA gene amplicon sequencing read count in SARS-CoV-2 positive floor samples showing no correlation with SARS-CoV-2 viral load. (B) Ct value of human RNAse P in SARS-CoV-2 positive floor samples showing significant correlation with SARS-CoV-2 viral load. Statistical analysis of scatter plots represents Pearson correlation, and box plots represents independent t-tests; *p < 0.05, **p < 0.01, ***p < 0.001. The legend in panel B applies to panel A as well. (C) Effect size of significant, non-redundant variables identified from Redundancy Analysis on unweighted UniFrac PCoA of floor samples.