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Transcriptomic profiling and functional prediction reveal aberrant expression of circular RNAs during osteogenic differentiation in human umbilical cord mesenchymal stromal cells
- Source :
- Scientific Reports, Vol 11, Iss 1, Pp 1-9 (2021), Scientific Reports
- Publication Year :
- 2021
- Publisher :
- Nature Portfolio, 2021.
-
Abstract
- Circular RNAs (circRNAs) are crucial elements of non-coding RNA, that regulate various biological processes. To date, expression patterns and functional roles of circRNAs during osteogenic differentiation of human umbilical cord mesenchymal stromal cells (hUCMSCs) remain unknown. In this study, we analyzed RNA-sequence data to reveal expression profiles of circRNAs during osteogenesis of hUCMSCs, then elucidated the underlying mechanisms of action. We identified a total of 5457 circRNAs in hUCMSCs, of which 34 and 33 were upregulated and downregulated, respectively. We applied Gene Ontology and Kyoto Encyclopedia of Genes and Genomes analyses to determine functions and related pathways of differentially expressed circRNAs. Moreover, we applied bioinformatics tools to construct competing endogenous RNA networks, comprising 10 circRNAs, 46 micro RNAs and 413 mRNAs. Furthermore, we predicted protein-coding potential of the upregulated circRNAs then constructed a co-expression network comprising the top 5 upregulated circRNAs and 75 RNA-binding proteins. Next, we validated 6 differentially-expressed circRNAs and found that overexpressing circ‐CTTN could promote osteogenesis of hUCMSCs. Overall, our findings indicate that clusters of circRNAs are aberrantly expressed in hUCMSCs during osteogenic differentiation, hence lay a foundation for future research into promoting hUCMSCs osteogenic differentiation and bone regeneration.
- Subjects :
- Science
Biology
Genome informatics
Umbilical cord
Article
Umbilical Cord
Transcriptome
Downregulation and upregulation
Osteogenesis
medicine
Humans
Gene Regulatory Networks
RNA, Messenger
KEGG
Bone regeneration
Cells, Cultured
Multidisciplinary
Competing endogenous RNA
Gene Expression Profiling
Mesenchymal stem cell
RNA
Computational Biology
High-Throughput Nucleotide Sequencing
Cell Differentiation
Mesenchymal Stem Cells
RNA, Circular
Cell biology
Stem-cell research
MicroRNAs
medicine.anatomical_structure
Gene Ontology
Gene Expression Regulation
Differentiation
Medicine
Subjects
Details
- Language :
- English
- ISSN :
- 20452322
- Volume :
- 11
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Scientific Reports
- Accession number :
- edsair.doi.dedup.....9366c538a75ad1aa169f08aecfa2ac57