Prevention of renal ischemia/reperfusion injury in rats using acetylcysteine after anesthesia with isoflurane

Submitted by Guilherme Lemeszenski (guilherme@nead.unesp.br) on 2013-08-22T18:58:17Z No. of bitstreams: 1 S0102-86502012000400010.pdf: 413885 bytes, checksum: 12b1c7e37e34fa549fdcc02d5f9eedab (MD5) Made available in DSpace on 2013-08-22T18:58:17Z (GMT). No. of bitstreams: 1 S0102-86502012000400010.pdf: 413885 bytes, checksum: 12b1c7e37e34fa549fdcc02d5f9eedab (MD5) Previous issue date: 2012-04-01 Made available in DSpace on 2013-09-30T19:53:51Z (GMT). No. of bitstreams: 2 S0102-86502012000400010.pdf: 413885 bytes, checksum: 12b1c7e37e34fa549fdcc02d5f9eedab (MD5) S0102-86502012000400010.pdf.txt: 25370 bytes, checksum: 02f3f0186a55643caefc3c74fde5697b (MD5) Previous issue date: 2012-04-01 Submitted by Vitor Silverio Rodrigues (vitorsrodrigues@reitoria.unesp.br) on 2014-05-20T13:31:57Z No. of bitstreams: 2 S0102-86502012000400010.pdf: 413885 bytes, checksum: 12b1c7e37e34fa549fdcc02d5f9eedab (MD5) S0102-86502012000400010.pdf.txt: 25370 bytes, checksum: 02f3f0186a55643caefc3c74fde5697b (MD5) Made available in DSpace on 2014-05-20T13:31:57Z (GMT). No. of bitstreams: 2 S0102-86502012000400010.pdf: 413885 bytes, checksum: 12b1c7e37e34fa549fdcc02d5f9eedab (MD5) S0102-86502012000400010.pdf.txt: 25370 bytes, checksum: 02f3f0186a55643caefc3c74fde5697b (MD5) Previous issue date: 2012-04-01 OBJETIVO: Avaliar o efeito da N-acetilcisteína na proteção renal contra lesão de isquemia/reperfusão, quando administrada logo após a indução anestésica, em ratos anestesiados com isoflurano. MÉTODOS: Dezoito ratos Wistar machos pesando mais que 300g foram anestesiados com isoflurano. A jugular interna direita e a carótida esquerda foram dissecadas e canuladas. Os animais foram distribuídos aleatoriamente em GAcetil, recebendo N-acetilcisteína por via intravenosa, 300mg/kg, e GIsot, solução salina. Foi realizada nefrectomia direita e clampeamento da artéria renal esquerda por 45 min. Os animais foram sacrificados após 48h, sendo colhidas amostras sanguíneas após a indução anestésica e ao sacrifício dos mesmos para avaliar a creatinina sérica. Realizou-se histologia renal. RESULTADOS: A variação da creatinina foi 2,33mg/dL ± 2,21 no GAcetil e 4,38mg/dL ± 2,13 no GIsot (p=0,074). Dois animais apresentaram necrose tubular intensa no GAcetil, comparados a cinco no GIsot. Apenas GAcetil apresentou animais livres de necrose tubular (dois) e degeneração tubular (um). CONCLUSÃO: Após isquemia/reperfusão renais, os ratos aos quais se administrou N-acetilcisteína apresentaram menor variação na creatinina sérica e lesões renais mais leves que o grupo controle. PURPOSE: To evaluate the effect of N-acetylcysteine, as a renoprotective agent, when administered early after anesthesia induction, against ischemia/reperfusion injury in rats anesthetized with isoflurane. METHODS: Eighteen male Wistar rats weighing > 300g were anesthetized with isoflurane. The internal jugular vein and the left carotid artery were dissected and cannulated. The animals were randomly divided into GAcetyl, receiving intravenous N-acetylcysteine, 300mg/kg, and GIsot, isotonic saline. After 30 minutes, right nephrectomy was performed and the left renal artery was clamped during 45 minutes. The animals were sacrificed after 48 hours and blood samples were taken after anesthetic induction and upon sacrificing of the animals to evaluate blood creatinine. The kidneys were sent for histological analysis. RESULTS: The variation in serum creatinine was 2.33mg/dL ± 2.21 in GAcetyl and 4.38mg/dL ± 2.13 in GIsot (p=0.074). Two animals presented intense tubular necrosis in GAcetyl, compared to 5 in GIsot. Only GAcetyl presented animals free of tubular necrosis (two) and tubular degeneration (one). CONCLUSION: After renal ischemia/reperfusion, the rats which were given N-acetylcysteine presented less variation in serum creatinine and milder kidney injuries than the control group. UNESP BSM Postgraduate Program in Anesthesiology UNESP BSM Department of Anesthesiology UNESP BSM Department of Pathology UNESP BSM Postgraduate Program in Anesthesiology UNESP BSM Department of Anesthesiology UNESP BSM Department of Pathology