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The Pleotrophic Effects of Insulin-Like Growth Factor-I on Human Spinal Cord Neural Progenitor Cells
- Source :
- Stem Cells and Development. 19:1983-1993
- Publication Year :
- 2010
- Publisher :
- Mary Ann Liebert Inc, 2010.
-
Abstract
- Most stem cell therapies involve direct, intraparachymal placement of neural progenitor cells. These cells provide physical support to the endogenous neuronal population and may be engineered to provide in situ growth factor support. Insulin-like growth factor-I (IGF-I) has potent neurotrophic and neuroprotective properties and is expressed by human neural stem cells (hNSCs). IGF-I is implicated in multiple aspects of cell behavior, including proliferation, differentiation, and survival. Enhancing hNSC function through IGF-I overexpression may increase the benefits of stem cell therapy. As a first step to that goal, we examined the direct effects of IGF-I on hNSC behavior in vitro. We demonstrate that IGF-I treatment enhances both the number and length of hNSC neurites. This is correlated with a decrease in proliferation, suggesting that IGF-I promotes neurite outgrowth but not proliferation. While IGF-I activates both AKT and MAPK signaling in hNSCs, we demonstrate that IGF-I-mediated neurite outgrowth is dependent only on AKT signaling. Finally, we demonstrate that IGF-I is neuroprotective after glutamate exposure in a model of excitotoxic cell death.
- Subjects :
- Neurite
Neurogenesis
medicine.medical_treatment
Blotting, Western
Cell
Fluorescent Antibody Technique
Glutamic Acid
Biology
Polymerase Chain Reaction
Neural Stem Cells
In Situ Nick-End Labeling
Neurites
medicine
Humans
Insulin-Like Growth Factor I
Protein kinase B
Cells, Cultured
Cell Proliferation
Cell Death
Growth factor
Cell Biology
Hematology
Stem-cell therapy
Neural stem cell
Cell biology
medicine.anatomical_structure
Spinal Cord
biology.protein
Mitogen-Activated Protein Kinases
Stem cell
Proto-Oncogene Proteins c-akt
Signal Transduction
Developmental Biology
Neurotrophin
Subjects
Details
- ISSN :
- 15578534 and 15473287
- Volume :
- 19
- Database :
- OpenAIRE
- Journal :
- Stem Cells and Development
- Accession number :
- edsair.doi.dedup.....93d4de6f1f2152e44fdb51e3e69c7d37