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Lgl mediates apical domain disassembly by suppressing the PAR-3-aPKC-PAR-6 complex to orient apical membrane polarity
- Source :
- Journal of Cell Science. 119:2107-2118
- Publication Year :
- 2006
- Publisher :
- The Company of Biologists, 2006.
-
Abstract
- The basolateral tumor suppressor protein Lgl is important for the regulation of epithelial cell polarity and tissue morphology. Recent studies have shown a physical and functional interaction of Lgl with another polarity-regulating protein machinery, the apical PAR-3-aPKC-PAR-6 complex, in epithelial cells. However, the mechanism of Lgl-mediated regulation of epithelial cell polarity remains obscure. By an siRNA method, we here show that endogenous Lgl is required for the disassembly of apical membrane domains in depolarizing MDCK cells induced by Ca2+ depletion. Importantly, this Lgl function is mediated by the suppression of the apical PAR-3-aPKC-PAR-6 complex activity. Analysis using 2D- or 3D-cultured cells in collagen gel suggests the importance of this suppressive regulation of Lgl on the collagen-mediated re-establishment of apical membrane domains and lumen formation. These results indicate that basolateral Lgl plays a crucial role in the disassembly of apical membrane domains to induce the orientation of apical membrane polarity, which is mediated by the suppression of apical PAR-3-aPKC-PAR-6 complex activity.
- Subjects :
- chemical and pharmacologic phenomena
Biology
Cell Line
Mice
Dogs
Membrane Microdomains
Cell polarity
Animals
RNA, Small Interfering
Protein Kinase C
Protein kinase C
Epithelial polarity
Homeodomain Proteins
Polarity (international relations)
Tumor Suppressor Proteins
Cell Polarity
Membrane Proteins
Proteins
Epithelial Cells
Depolarization
Cell Biology
Apical membrane
Cell biology
Cytoskeletal Proteins
Membrane protein
Cell culture
Receptors, Thrombin
Collagen
Subjects
Details
- ISSN :
- 14779137 and 00219533
- Volume :
- 119
- Database :
- OpenAIRE
- Journal :
- Journal of Cell Science
- Accession number :
- edsair.doi.dedup.....93db50a029f6a9692a9b35731928d905