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Genome-wide identification and expression analysis of anthocyanin biosynthetic genes in Brassica juncea
- Source :
- Journal of Integrative Agriculture, Vol 19, Iss 5, Pp 1250-1260 (2020)
- Publication Year :
- 2020
- Publisher :
- Elsevier BV, 2020.
-
Abstract
- Anthocyanins confer the wide range of colors for plants and also play beneficial health roles as potentially protective factors against heart disease and cancer. Brassica juncea is cultivated as an edible oil resource and vegetable crop worldwide, thus elucidating the anthocyanin biosynthetic pathway would be helpful to improve the nutritional quality of Brassica juncea through the breeding and cultivating of high anthocyanin content varieties. Herein, 129 genes in B. juncea were identified as orthologs of 41 anthocyanin biosynthetic genes (ABGs) in Arabidopsis thaliana by comparative genomic analyses. The B. juncea ABGs have expanded by whole genome triplication and subsequent allopolyploidizatoin, but lost mainly during the whole genome triplication between B. rapa/B. nigra and A. thaliana, rather than the allopolyploidization process between B. juncea and B. rapa/B. nigra, leading to different copy numbers retention of A. thaliana homologous genes. Although the overall expansion levels ABGs were similar to the whole genome, more negative regulatory genes were retained in the anthocyanin biosynthesis regulatory system. Transcriptional analysis of B. juncea with different anthocyanin accumulation showed that BjDFR, BjTT19, BjTT8 are significantly up-regulated in plants with purple leaves as compared with green leaves. The overexpression of BjTT8 and these target genes which were involved in late anthocyanin biosynthesis and transport might account for increasing levels of anthocyanin accumulation in purple leaves. Our results could promote the understanding of the genetic mechanism of anthocyanin biosynthesis in B. juncea.
- Subjects :
- 0106 biological sciences
Agriculture (General)
Brassica
Plant Science
01 natural sciences
Biochemistry
Genome
S1-972
chemistry.chemical_compound
Food Animals
Expression analysis
Arabidopsis thaliana
comparative genomic analysis
Gene
Regulator gene
Genetics
Ecology
biology
fungi
food and beverages
04 agricultural and veterinary sciences
leaf color
biology.organism_classification
chemistry
Anthocyanin
040103 agronomy & agriculture
0401 agriculture, forestry, and fisheries
anthocyanin biosynthetic genes
Animal Science and Zoology
B. juncea
transcriptome
Agronomy and Crop Science
010606 plant biology & botany
Food Science
Biosynthetic genes
Subjects
Details
- ISSN :
- 20953119
- Volume :
- 19
- Database :
- OpenAIRE
- Journal :
- Journal of Integrative Agriculture
- Accession number :
- edsair.doi.dedup.....94045e515c0fe714afee8e62da271ae6