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Proteomics reveals disturbances in the immune response and energy metabolism of monocytes from patients with septic shock

Authors :
Giselle Villa Flor Brunoro
Helder I. Nakaya
Monique R.O. Trugilho
Patrícia T. Bozza
Richard H. Valente
Fernando A. Bozza
Pedro Mendes de Azambuja Rodrigues
Mariana Araujo-Pereira
Source :
Scientific Reports, Vol 11, Iss 1, Pp 1-10 (2021), Scientific Reports, Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual), Universidade de São Paulo (USP), instacron:USP
Publication Year :
2021
Publisher :
Nature Portfolio, 2021.

Abstract

Sepsis results from a dyshomeostatic response to infection, which may lead to hyper or hypoimmune states. Monocytes are central regulators of the inflammatory response, but our understanding of their role in the genesis and resolution of sepsis is still limited. Here, we report a comprehensive exploration of monocyte molecular responses in a cohort of patients with septic shock via proteomic profiling. The acute stage of septic shock was associated with an impaired inflammatory phenotype, indicated by the down-regulation of MHC class II molecules and proinflammatory cytokine pathways. Simultaneously, there was an up-regulation of glycolysis enzymes and a decrease in proteins related to the citric acid cycle and oxidative phosphorylation. On the other hand, the restoration of immunocompetence was the hallmark of recovering patients, in which an upregulation of interferon signaling pathways was a notable feature. Our results provide insights into the immunopathology of sepsis and propose that, pending future studies, immunometabolism pathway components could serve as therapeutic targets in septic patients.

Details

Language :
English
ISSN :
20452322
Volume :
11
Issue :
1
Database :
OpenAIRE
Journal :
Scientific Reports
Accession number :
edsair.doi.dedup.....940ee140554d23cdfdd9c0feffaf7ffe