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Anti-inflammatory and antiarthritic effects of piperine in human interleukin 1β-stimulated fibroblast-like synoviocytes and in rat arthritis models
Anti-inflammatory and antiarthritic effects of piperine in human interleukin 1β-stimulated fibroblast-like synoviocytes and in rat arthritis models
- Source :
- Arthritis Research & Therapy
- Publication Year :
- 2009
- Publisher :
- Springer Science and Business Media LLC, 2009.
-
Abstract
- Introduction The objective of this study was to determine the anti-inflammatory, nociceptive, and antiarthritic effects of piperine, the active phenolic component in black pepper extract. Methods The in vitro anti-inflammatory activity of piperine was tested on interleukin 1β (IL1β)-stimulated fibroblast-like synoviocytes derived form patients with rheumatoid arthritis. The levels of IL6, matrix metalloproteinase (MMPs), cyclo-oxygenase 2 (COX-2), and prostaglandin E2 (PGE2) were investigated by ELISA and RT-PCR analysis. The analgesic and antiarthritic activities of piperine were investigated on rat models of carrageenan-induced acute paw pain and arthritis. The former were evaluated with a paw pressure test, and the latter by measuring the squeaking score, paw volume, and weight distribution ratio. Piperine was administrated orally to rats at 20 and 100 mg/kg/day for 8 days. Results Piperine inhibited the expression of IL6 and MMP13 and reduced the production of PGE2 in a dose dependant manner at concentrations of 10 to 100 μg/ml. In particular, the production of PGE2 was significantly inhibited even at 10 μg/ml of piperine. Piperine inhibited the migration of activator protein 1 (AP-1), but not nuclear factor (NF)κB, into the nucleus in IL1β-treated synoviocytes. In rats, piperine significantly reduced nociceptive and arthritic symptoms at days 8 and 4, respectively. Histological staining showed that piperine significantly reduced the inflammatory area in the ankle joints. Conclusions These results suggest that piperine has anti-inflammatory, antinociceptive, and antiarthritic effects in an arthritis animal model. Thus, piperine should be further studied with regard to use either as a pharmaceutical or as a dietary supplement for the treatment of arthritis.
- Subjects :
- Male
Polyunsaturated Alkamides
medicine.drug_class
Interleukin-1beta
Immunology
Anti-Inflammatory Agents
Arthritis
Enzyme-Linked Immunosorbent Assay
Inflammation
Matrix metalloproteinase
Pharmacology
Dinoprostone
Anti-inflammatory
Rats, Sprague-Dawley
chemistry.chemical_compound
Alkaloids
Piperidines
Rheumatology
Animals
Humans
Immunology and Allergy
Medicine
Benzodioxoles
Fibroblast
Interleukin 6
biology
Interleukin-6
Reverse Transcriptase Polymerase Chain Reaction
business.industry
Synovial Membrane
food and beverages
Fibroblasts
medicine.disease
Arthritis, Experimental
Matrix Metalloproteinases
Rats
medicine.anatomical_structure
chemistry
Cyclooxygenase 2
Hyperalgesia
Piperine
biology.protein
Synovial membrane
medicine.symptom
business
Research Article
Subjects
Details
- ISSN :
- 14786354
- Volume :
- 11
- Database :
- OpenAIRE
- Journal :
- Arthritis Research & Therapy
- Accession number :
- edsair.doi.dedup.....942780914827b331665e42d23a50c7bc
- Full Text :
- https://doi.org/10.1186/ar2662