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AIR POLLUTION INDUCES ENHANCED MITOCHONDRIAL OXIDATIVE STRESS IN CYSTIC FIBROSIS AIRWAY EPITHELIUM
- Publication Year :
- 2008
-
Abstract
- We studied the effects of airborne particulate matters (PM) on cystic fibrosis (CF) epithelium. We noted that PM enhanced human CF bronchial epithelial apoptosis, activated caspase-9 and PARP-1; and reduced mitochondrial membrane potential. Mitochondrial inhibitors (4,4-diisothiocyanatostilbene-2,2′disulfonic acid, rotenone and thenoyltrifluoroacetone) blocked PM-induced generation of reactive oxygen species and apoptosis. PM upregulated pro-apoptotic Bad, Bax, p53 and p21; and enhanced mitochondrial localization of Bax. The anti-apoptotic Bcl-2, Bcl-xl, Mcl-1 and Xiap remained unchanged; however, overexpression of Bcl-xl blocked PM-induced apoptosis. Accordingly, we provide the evidence that PM enhances oxidative stress and mitochondrial signaling mediated apoptosis via the modulation of Bcl family proteins in CF.
- Subjects :
- Ambient air pollution particle
Cystic Fibrosis
Biophysics
Apoptosis
Bronchi
Respiratory Mucosa
Mitochondrion
Biology
medicine.disease_cause
Biochemistry
Article
Epithelium
Cell Line
Structural Biology
Air Pollution
Genetics
medicine
Humans
Molecular Biology
chemistry.chemical_classification
Reactive oxygen species
Cell Biology
Cystic fibrosis transmembrane conductance regulator
Cell biology
XIAP
Mitochondria
Oxidative Stress
medicine.anatomical_structure
chemistry
biology.protein
Respiratory epithelium
Particulate Matter
Apoptosis Regulatory Proteins
Oxidative stress
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Accession number :
- edsair.doi.dedup.....945da384ca9930f40a299c47f02ea9fb