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Low level laser therapy increases angiogenesis in a model of ischemic skin flap in rats mediated by VEGF, HIF-1α and MMP-2*
- Source :
- Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual), Universidade de São Paulo (USP), instacron:USP
- Publication Year :
- 2013
-
Abstract
- It is known that low level laser therapy is able to improve skin flap viability by increasing angiogenesis. However, the mechanism for new blood vessel formation is not completely understood. Here, we investigated the effects of 660 nm and 780 nm lasers at fluences of 30 and 40 J/cm(2) on three important mediators activated during angiogenesis. Sixty male Wistar rats were used and randomly divided into five groups with twelve animals each. Groups were distributed as follows: skin flap surgery non-irradiated group as a control; skin flap surgery irradiated with 660 nm laser at a fluence of 30 or 40 J/cm(2) and skin flap surgery irradiated with 780 nm laser at a fluence of 30 or 40 J/cm(2). The random skin flap was performed measuring 10×4 cm, with a plastic sheet interposed between the flap and the donor site. Laser irradiation was performed on 24 points covering the flap and surrounding skin immediately after the surgery and for 7 consecutive days thereafter. Tissues were collected, and the number of vessels, angiogenesis markers (vascular endothelial growth factor, VEGF and hypoxia inducible factor, HIF-1α) and a tissue remodeling marker (matrix metalloproteinase, MMP-2) were analyzed. LLLT increased an angiogenesis, HIF-1α and VEGF expression and decrease MMP-2 activity. These phenomena were dependent on the fluences, and wavelengths used. In this study we showed that LLLT may improve the healing of skin flaps by enhancing the amount of new vessels formed in the tissue. Both 660 nm and 780 nm lasers were able to modulate VEGF secretion, MMP-2 activity and HIF-1α expression in a dose dependent manner.
- Subjects :
- Male
Vascular Endothelial Growth Factor A
Pathology
medicine.medical_specialty
Angiogenesis
medicine.medical_treatment
Biophysics
Ischemia
Neovascularization, Physiologic
Matrix metalloproteinase
Article
Surgical Flaps
RATOS
chemistry.chemical_compound
medicine
Animals
Radiology, Nuclear Medicine and imaging
Low-Level Light Therapy
Rats, Wistar
Low level laser therapy
Skin
Radiation
Radiological and Ultrasound Technology
Anatomy
medicine.disease
Hypoxia-Inducible Factor 1, alpha Subunit
Rats
Vascular endothelial growth factor
Vascular endothelial growth factor A
medicine.anatomical_structure
chemistry
Hypoxia-inducible factors
Matrix Metalloproteinase 2
Blood vessel
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Journal :
- Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual), Universidade de São Paulo (USP), instacron:USP
- Accession number :
- edsair.doi.dedup.....94621fecf4e5fc6fd3ce25bd5a68743d