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Molecular detection and characterization of Carnivore chaphamaparvovirus 1 in dogs
- Source :
- Veterinary Microbiology
- Publication Year :
- 2020
- Publisher :
- Elsevier BV, 2020.
-
Abstract
- Canine chaphamaparvovirus (CaChPV) is a newly recognised parvovirus discovered by metagenomic analysis during an outbreak of diarrhoea in dogs in Colorado, USA, in 2017 and more recently detected in diarrhoeic dogs in China. Whether the virus plays a role as canine pathogen and whether it is distributed elsewhere, in other geographical areas, is not known. We performed a case-control study to investigate the possible association of CaChPV with enteritis in dogs. CaChPV DNA was detected both in the stools of diarrhoeic dogs (1.9 %, 3/155) and of healthy animals (1.6 %, 2/120). All the CaChPV-infected dogs with diarrhea were mixed infected with other enteric viruses such as canine parvovirus (formerly CPV-2), canine bufavirus (CBuV) and canine coronavirus (CCoV), whilst none of the asymptomatic CaChPV positive animals resulted co-infected. The nearly full-length genome and the partial capsid protein (VP) gene of three canine strains, Te/36OVUD/19/ITA, Te/37OVUD/19/ITA and Te/70OVUD/19/ITA, were reconstructed. Upon phylogenetic analyses based on the NS1 and VP aa sequences, the Italian CaChPV strains tightly clustered with the American reference viruses. Distinctive residues could be mapped to the deduced variable regions of the VP of canine and feline chaphamaparvoviruses, considered as important markers of host range and pathogenicity for parvoviruses.
- Subjects :
- Diarrhea
Enteric samples
Parvovirus, Canine
viruses
Genome, Viral
Viral Nonstructural Proteins
Microbiology
Virus
Host Specificity
Parvoviridae
Enteritis
Parvoviridae Infections
03 medical and health sciences
Feces
Dogs
medicine
Animals
Dog Diseases
Pathogen
Phylogeny
030304 developmental biology
Canine chaphamaparvovirus 1
0303 health sciences
biology
General Veterinary
030306 microbiology
Parvovirus
Canine parvovirus
Outbreak
Canine coronavirus
Pets
General Medicine
biology.organism_classification
medicine.disease
Virology
Italy
Case-Control Studies
Capsid Proteins
Subjects
Details
- Language :
- English
- ISSN :
- 03781135
- Database :
- OpenAIRE
- Journal :
- Veterinary Microbiology
- Accession number :
- edsair.doi.dedup.....947044f2d2d5fb65320b9131ad19cd00
- Full Text :
- https://doi.org/10.1016/j.vetmic.2020.108878