Back to Search
Start Over
Defining the genome content of live plague vaccines by use of whole-genome DNA microarray
- Source :
- Vaccine. 22(25-26)
- Publication Year :
- 2003
-
Abstract
- Yersinia pestis whole-genome DNA microarrays were developed to perform genomic comparison of a collection of live plague vaccines. By using the genomic DNA to probe the DNA microarrays, we detected dozens of deletions and amplifications of the genomic regions in the 19 vaccine strains analyzed. The revealed genomic differences within the vaccine strains of different origins provide us an unprecedented opportunity to understand the molecular background of the variability of the immunogenic and protective potency of plague live vaccine. The whole-genome DNA microarray also provides an ideal tool to perform the pre-evaluation of a vaccine strain for its high throughput to determine the genomic features essential or unallowable for the live vaccines.
- Subjects :
- DNA, Bacterial
Yersinia pestis
Biology
Plague (disease)
Vaccines, Attenuated
Genome
Vaccine strain
medicine
Humans
Oligonucleotide Array Sequence Analysis
Genetics
Plague
Plague Vaccine
Attenuated vaccine
General Veterinary
General Immunology and Microbiology
Reverse Transcriptase Polymerase Chain Reaction
Public Health, Environmental and Occupational Health
Yersiniosis
Chromosomes, Bacterial
medicine.disease
biology.organism_classification
genomic DNA
Infectious Diseases
Molecular Medicine
DNA microarray
Genome, Bacterial
Plasmids
Subjects
Details
- ISSN :
- 0264410X
- Volume :
- 22
- Issue :
- 25-26
- Database :
- OpenAIRE
- Journal :
- Vaccine
- Accession number :
- edsair.doi.dedup.....94950095156f998f6a4dfeba4eb3c41a