Back to Search
Start Over
CRISPR-Cas13a Inhibits HIV-1 Infection
- Source :
- Molecular Therapy. Nucleic Acids, Molecular Therapy: Nucleic Acids, Vol 21, Iss, Pp 147-155 (2020)
- Publication Year :
- 2020
- Publisher :
- Elsevier BV, 2020.
-
Abstract
- CRISPR-Cas provides bacteria and archaea with immunity against invading phages and foreign plasmid DNA and has been successfully adapted for gene editing in a variety of species. The class 2 type VI CRISPR-Cas effector Cas13a targets and cleaves RNA, providing protection against RNA phages. Here we report the repurposing of CRISPR-Cas13a to inhibit human immunodeficiency virus type 1 (HIV-1) infection through targeting HIV-1 RNA and diminishing viral gene expression. We observed strong inhibition of HIV-1 infection by CRISPR-Cas13a in human cells. We showed that CRISPR-Cas13a not only diminishes the level of newly synthesized viral RNA, either from the transfected plasmid DNA or from the viral DNA, which is integrated into cellular DNA, but it also targets and destroys the viral RNA that enters cells within viral capsid, leading to strong inhibition of HIV-1 infection. Together, our results suggest that CRISPR-Cas13a provides a potential novel tool to treat viral diseases in humans.<br />Graphical Abstract<br />The class 2 type VI CRISPR-Cas effector Cas13a targets and cleaves RNA, providing protection against RNA phages. Yin et al. demonstrate that CRISPR-Cas13a targets and cleaves the HIV RNA genome and mRNA, leading to strong inhibition of HIV-1 infection.
- Subjects :
- 0301 basic medicine
Effector
viruses
lcsh:RM1-950
RNA
Transfection
RNA Phages
Biology
Virology
Article
3. Good health
03 medical and health sciences
lcsh:Therapeutics. Pharmacology
030104 developmental biology
0302 clinical medicine
Genome editing
Capsid
Immunity
030220 oncology & carcinogenesis
Drug Discovery
Molecular Medicine
CRISPR
Subjects
Details
- ISSN :
- 21622531
- Volume :
- 21
- Database :
- OpenAIRE
- Journal :
- Molecular Therapy - Nucleic Acids
- Accession number :
- edsair.doi.dedup.....94a0d50a5f8da4468d3af933453e33e7