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Inhibitory effects of beer on heterocyclic amine-induced mutagenesis and PhIP-induced aberrant crypt foci in rat colon
- Source :
- Mutation research. 559(1-2)
- Publication Year :
- 2003
-
Abstract
- Anti-mutagenic and anti-carcinogenic effects of beer on heterocyclic amine (HCA)-induced carcinogenesis were studied in vitro and in vivo. Four commercial beers (two pilsner-type, black, and stout) showed inhibitory effects against five HCAs, 2-amino-3,8-dimethylimidazo [4,5-f]quinoxaline (MeIQx), 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP), 3-amino-1-methyl-5H-pyrido[4,3-b]indole (Trp-P-2), 2-amino-6-methyldipyrido[1,2-a:3',2'-d]imidazole (Glu-P-1) and 2-amino-3-methylimidazo[4,5-f]-quinoline (IQ), in the Ames assay using Salmonella typhimurium TA98 in the presence of rat S9 mix. The inhibitory effects of dark-colored beers (stout and black beer) were greater than those of pilsner-type beers. Dark-colored beers suppressed CYP1A2 activity in a dose-dependent manner, suggesting that inhibition of HCA activation is partly responsible for their strong anti-mutagenic effects. Anti-mutagenic effects were also observed when the pooled human S9 mix or activated IQ was used in the assay. The micronucleus test using Chinese hamster lung CHL/IU cells showed that the addition of freeze-dried samples of pilsner-type and stout beer to the culture medium significantly reduced the number of cells with micronuclei induced with PhIP or Trp-P-2. Single-cell gel electrophoresis assay (comet assay) revealed that oral ingestion of pilsner-type and stout beers for 1 week significantly inhibited DNA damage in the liver cells of male ICR mice exposed to MeIQx (13 mg/kg, i.p.). A decrease in the formation of DNA adducts was also observed using a 32P-postlabeling method. Male Fischer 344 rats orally received PhIP (75 mg/kg, five times a week for 2 weeks) and aberrant crypt foci (ACF) formation in the colon was analyzed after 5 weeks. The number of ACF was significantly reduced in rats fed a diet containing freeze-dried beer. These results suggest that beer inhibits the genotoxic effects of HCAs and may reduce the risk of carcinogenesis caused by food borne carcinogens.
- Subjects :
- Salmonella typhimurium
Colon
Health, Toxicology and Mutagenesis
Ames test
chemistry.chemical_compound
DNA Adducts
Cricetulus
Cytochrome P-450 Enzyme System
In vivo
Cytochrome P-450 CYP1A2
Heterocyclic Compounds
Cricetinae
Genetics
Animals
Amines
Carcinogen
Cells, Cultured
chemistry.chemical_classification
2-Amino-1-methyl-6-phenylimidazo(4,5-b)pyridine
Micronucleus Tests
Dose-Response Relationship, Drug
food and beverages
Beer
Antimutagenic Agents
Molecular biology
Rats
Comet assay
Spectrometry, Fluorescence
Biochemistry
chemistry
Liver
Mutagenesis
Heterocyclic amine
Micronucleus test
Comet Assay
Oxidoreductases
Aberrant crypt foci
DNA Damage
Subjects
Details
- ISSN :
- 00275107
- Volume :
- 559
- Issue :
- 1-2
- Database :
- OpenAIRE
- Journal :
- Mutation research
- Accession number :
- edsair.doi.dedup.....94a16a3d3ab2b75dc3e9cef8fe9e25e9