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A double-armed, hydrophilic transition metal complex as paramagnetic NMR probe
- Source :
- Angewandte Chemie International Edition, Angewandte Chemie International Edition, 58(37), 13093-13100, Angewandte Chemie (International Ed. in English)
- Publication Year :
- 2019
-
Abstract
- Synthetic metal complexes can be used as paramagnetic probes for the study of proteins and protein complexes. Herein, two transition metal NMR probes (TraNPs) are reported. TraNPs are attached through two arms to a protein to generate a pseudocontact shift (PCS) using cobalt(II), or paramagnetic relaxation enhancement (PRE) with manganese(II). The PCS analysis of TraNPs attached to three different proteins shows that the size of the anisotropic component of the magnetic susceptibility depends on the probe surroundings at the surface of the protein, contrary to what is observed for lanthanoid‐based probes. The observed PCS are relatively small, making cobalt‐based probes suitable for localized studies, such as of an active site. The obtained PREs are stronger than those obtained with nitroxide spin labels and the possibility to generate both PCS and PRE offers advantages. The properties of TraNPs in comparison with other cobalt‐based probes are discussed.
- Subjects :
- Lanthanide
Nitroxide mediated radical polymerization
Materials science
chemistry.chemical_element
010402 general chemistry
NMR Spectroscopy
01 natural sciences
magnetic susceptibility tensors
transition metals
Catalysis
Metal
Paramagnetism
Transition metal
Research Articles
010405 organic chemistry
General Medicine
General Chemistry
Nuclear magnetic resonance spectroscopy
Magnetic susceptibility
paramagnetic relaxation enhancements
0104 chemical sciences
Crystallography
chemistry
visual_art
pseudocontact shifts
visual_art.visual_art_medium
Cobalt
Research Article
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Journal :
- Angewandte Chemie International Edition, Angewandte Chemie International Edition, 58(37), 13093-13100, Angewandte Chemie (International Ed. in English)
- Accession number :
- edsair.doi.dedup.....94acf4ed2de28a1d4d5d31587a931597