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How to image cell adhesion on soft polymers?
- Source :
- Micron 92, 39-42 (2017). doi:10.1016/j.micron.2016.11.002
- Publication Year :
- 2017
- Publisher :
- Elsevier BV, 2017.
-
Abstract
- Here, we present a method to investigate cell adhesion on soft, non-conducting polymers that are implant candidate materials. Neuronal cells were grown on two elastomers (polydimethylsiloxane (PDMS) and Ecoflex®) and prepared for electron microscopy. The samples were treated with osmium tetroxide (OsO4) and uranylacetate (UrAc). Best results can be achieved when the polymers were coated with a thin iridium layer before the cell culture. This was done to emphasize the usage of soft polymers as supports for implant electrodes. A good contrast and the adhesion of the cells on soft polymers could be visualized.
- Subjects :
- Materials science
Osmium Tetroxide
Polymers
Polyesters
Cell Culture Techniques
General Physics and Astronomy
Nanotechnology
02 engineering and technology
Iridium
010402 general chemistry
Elastomer
01 natural sciences
Specimen Handling
law.invention
chemistry.chemical_compound
Implants, Experimental
Structural Biology
law
ddc:570
Cell Adhesion
Organometallic Compounds
General Materials Science
Dimethylpolysiloxanes
Cell adhesion
Neurons
chemistry.chemical_classification
Polydimethylsiloxane
Cell Biology
Polymer
Adhesion
021001 nanoscience & nanotechnology
0104 chemical sciences
Microscopy, Electron
chemistry
Osmium tetroxide
Electron microscope
0210 nano-technology
Layer (electronics)
Biomedical engineering
Subjects
Details
- ISSN :
- 09684328
- Volume :
- 92
- Database :
- OpenAIRE
- Journal :
- Micron
- Accession number :
- edsair.doi.dedup.....94ce03c44b5262a34a75a27d70dbc2a8