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A LacI-Family Regulator Activates Maltodextrin Metabolism of Enterococcus faecium
- Source :
- PLoS ONE, PLoS ONE, Vol 8, Iss 8, p e72285 (2013), PLoS ONE; Vol 8
- Publication Year :
- 2013
- Publisher :
- Public Library of Science, 2013.
-
Abstract
- Enterococcus faecium is a gut commensal of humans and animals. In the intestinal tract, E. faecium will have access to a wide variety of carbohydrates, including maltodextrins and maltose, which are the sugars that result from the enzymatic digestion of starch by host-derived and microbial amylases. In this study, we identified the genetic determinants for maltodextrin utilization of E. faecium E1162. We generated a deletion mutant of the mdxABCD-pulA gene cluster that is homologous to maltodextrin uptake genes in other Gram-positive bacteria, and a deletion mutant of the mdxR gene, which is predicted to encode a LacI family regulator of mdxABCD-pulA. Both mutations impaired growth on maltodextrins but had no effect on the growth on maltose and glucose. Comparative transcriptome analysis showed that eight genes (including mdxABCD-pulA) were expressed at significantly lower levels in the isogenic ΔmdxR mutant strain compared to the parental strain when grown on maltose. Quantitative real-time RT-PCR confirmed the results of transcriptome analysis and showed that the transcription of a putative maltose utilization gene cluster is induced in a semi-defined medium supplemented with maltose but is not regulated by MdxR. Understanding the maltodextrin metabolism of E. faecium could yield novel insights into the underlying mechanisms that contribute to the gut commensal lifestyle of E. faecium.
- Subjects :
- Enterococcus faecium
lcsh:Medicine
Microbiology
Transcriptome
03 medical and health sciences
chemistry.chemical_compound
Bacterial Proteins
Polysaccharides
Gene cluster
Lac Repressors
Amylase
lcsh:Science
Maltose
Gene
030304 developmental biology
Oligonucleotide Array Sequence Analysis
0303 health sciences
Multidisciplinary
biology
030306 microbiology
Reverse Transcriptase Polymerase Chain Reaction
lcsh:R
food and beverages
Starch
Gene Expression Regulation, Bacterial
biology.organism_classification
Maltodextrin
chemistry
Biochemistry
Multigene Family
Mutation
biology.protein
lcsh:Q
Bacteria
Research Article
Subjects
Details
- Language :
- English
- ISSN :
- 19326203
- Volume :
- 8
- Issue :
- 8
- Database :
- OpenAIRE
- Journal :
- PLoS ONE
- Accession number :
- edsair.doi.dedup.....94d34ec3188e21403918ae85b0ee7b36