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Development of a fluorogenic small substrate for dipeptidyl peptidase-4
- Source :
- Beilstein Journal of Organic Chemistry, Beilstein Journal of Organic Chemistry, Vol 13, Iss 1, Pp 2690-2697 (2017)
- Publication Year :
- 2017
- Publisher :
- Beilstein-Institut, 2017.
-
Abstract
- A series of aniline and m-phenylenediamine derivatives with electron-withdrawing 3,3,3-trifluoropropenyl substituents were synthesized as small and chemically stable fluorescent organic compounds. Their fluorescence performances were evaluated by converting 2,4-disubstituted aniline 1 to the non-fluorescent dipeptide analogue H-Gly-Pro-1 for the use as a fluorogenic substrate for dipeptidyl peptidase-4 (DPP-4). The progress of the enzymatic hydrolysis of H-Gly-Pro-1 with DPP-4 was monitored by fluorometric determination of 1 released into the reaction medium. The results suggest that 1 could be used as fluorophore in OFF–ON-type fluorogenic probes.
- Subjects :
- dipeptidyl peptidase-4
Fluorophore
animal structures
010402 general chemistry
01 natural sciences
Fluorescence spectroscopy
Full Research Paper
lcsh:QD241-441
chemistry.chemical_compound
Aniline
lcsh:Organic chemistry
Enzymatic hydrolysis
lcsh:Science
Dipeptidyl peptidase-4
Dipeptide
010405 organic chemistry
Chemistry
Organic Chemistry
fluorometry
Substrate (chemistry)
Combinatorial chemistry
Fluorescence
0104 chemical sciences
small fluorescent molecule
fluorogenic substrate
lcsh:Q
Subjects
Details
- Language :
- English
- ISSN :
- 18605397
- Volume :
- 13
- Database :
- OpenAIRE
- Journal :
- Beilstein Journal of Organic Chemistry
- Accession number :
- edsair.doi.dedup.....9512c6d85de34a5d90b315090ebb1b91