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Growth inhibition of subcutaneously transplanted human glioma by transfection-induced tumor necrosis factor-? and augmentation of the effect by ?-interferon
- Source :
- Journal of Neuro-Oncology. 22:221-225
- Publication Year :
- 1994
- Publisher :
- Springer Science and Business Media LLC, 1994.
-
Abstract
- Using subcutaneous solid tumors produced by U251-MG human glioma cells, we studied the in vivo transfection of the cells with the tumor necrosis factor-alpha (TNF-alpha) gene delivered by means of liposomes. When the tumor had become 7 mm in diameter, liposomes with entrapped TNF-alpha gene were injected into the center of the subcutaneous tumor. We found that mRNA of transfection-induced TNF-alpha, which was expressed in the tumor tissue, was detected by reverse transcriptase-polymerase chain reaction (RT-PCR) method and its protein was demonstrated by enzyme-linked immunoassay. Growth of the tumor was inhibited when the injection was carried out five times at every other day. The growth-inhibitory effect by transfection-induced TNF-alpha was much remarkable as compared with exogenous TNF-alpha and the effect was enhanced by the intraperitoneal injection of gamma-interferon (IFN-gamma) 12 h prior to intratumoral injection of the liposomes.
- Subjects :
- Cancer Research
Time Factors
medicine.medical_treatment
Molecular Sequence Data
Intraperitoneal injection
Gene Expression
Mice, Nude
Biology
Transfection
Polymerase Chain Reaction
Cell Line
Interferon-gamma
Mice
chemistry.chemical_compound
Glioma
Tumor Cells, Cultured
medicine
Animals
Humans
RNA, Messenger
DNA Primers
Messenger RNA
Liposome
Base Sequence
medicine.diagnostic_test
Tumor Necrosis Factor-alpha
Genetic Therapy
medicine.disease
Combined Modality Therapy
Survival Analysis
Molecular biology
Neurology
Oncology
chemistry
Immunoassay
Liposomes
Female
Tumor necrosis factor alpha
Neurology (clinical)
Growth inhibition
Cell Division
Subjects
Details
- ISSN :
- 15737373 and 0167594X
- Volume :
- 22
- Database :
- OpenAIRE
- Journal :
- Journal of Neuro-Oncology
- Accession number :
- edsair.doi.dedup.....95258d528a2a1c968eca6b33e6f5890b