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Inhibition of the osteogenic differentiation of mesenchymal stem cells derived from the offspring of rats treated with caffeine during pregnancy and lactation
- Source :
- Connective Tissue Research. 57:131-142
- Publication Year :
- 2015
- Publisher :
- Informa UK Limited, 2015.
-
Abstract
- Caffeine is an alkaloid that is widely consumed due to its presence in drugs, coffee, tea, and chocolate. This compound passes to offspring through the placenta and milk; can cause teratogenic mutations; and reduces the formation, growth, and mass of bone. Because mesenchymal stem cells (MSCs) are responsible for generating the entire skeleton, we hypothesized that these cells are targets of caffeine. This study evaluated the osteogenic differentiation of MSCs derived from the offspring of rats treated with caffeine during pregnancy and lactation. Twenty-four adult Wistar rats were randomly divided into four groups, including one control group and three experimental groups treated with 25, 50, or 100 mg/kg of caffeine. At weaning, three 21-day-old pups from each dam in each group were euthanized for extraction of bone marrow cells for in vitro tests. Caffeine doses of 50 and 100 mg/kg significantly reduced the activity of alkaline phosphatase at 7, 14, and 21 days and the expression of collagen I at 21 days. However, the expression of gene transcripts for alkaline phosphatase, Runx-2, and bone sialoprotein, as well as the synthesis of mineralization nodules, decreased significantly in all groups treated with caffeine. The expression of osteocalcin was significantly reduced only in the group treated with 50 mg/kg caffeine. The caffeine that passes from the mother to the offspring during pregnancy and lactation reduces the osteogenic differentiation of MSCs. We propose that this reduction in the osteogenic potential of MSCs may be involved in the pathogenesis of osteopenia resulting from caffeine consumption.
- Subjects :
- 0301 basic medicine
medicine.medical_specialty
Offspring
Real-Time Polymerase Chain Reaction
Biochemistry
03 medical and health sciences
chemistry.chemical_compound
Calcification, Physiologic
Rheumatology
Antigens, CD
Osteogenesis
Pregnancy
Caffeine
Lactation
Internal medicine
Animals
Medicine
Orthopedics and Sports Medicine
Rats, Wistar
Molecular Biology
Cells, Cultured
biology
business.industry
Alkaloid
Mesenchymal stem cell
Cell Differentiation
Mesenchymal Stem Cells
Cell Biology
Alkaline Phosphatase
Biotechnology
Phenotype
030104 developmental biology
Endocrinology
medicine.anatomical_structure
chemistry
Osteocalcin
biology.protein
Alkaline phosphatase
Female
Bone marrow
business
Subjects
Details
- ISSN :
- 16078438 and 03008207
- Volume :
- 57
- Database :
- OpenAIRE
- Journal :
- Connective Tissue Research
- Accession number :
- edsair.doi.dedup.....954d26a7b53b320a446cba36caf3ab8e