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Identification and characterization of microRNAs in the gonad of Trachinotus ovatus using Solexa sequencing
- Source :
- Comparative Biochemistry and Physiology Part D: Genomics and Proteomics. 30:312-320
- Publication Year :
- 2019
- Publisher :
- Elsevier BV, 2019.
-
Abstract
- The Trachinotus ovatus (T. ovatus) reach sexual maturity as late as 4–5 years, and there are no obvious morphology differences between males and females, even at maturity, making the selection of male and female parents for selective breeding difficult. To examine the potential regulatory mechanism of sexual differentiation, we conducted a microRNAs (miRNAs) analysis on the ovaries and testes of T. ovatus. A total of 13,423,691 and 11,734,953 raw reads, representing 91,883,908 and 86,879,726 unique sequences of 18–35 nt length obtained from the ovaries and testes, respectively. After mapping to the T. ovatus transcriptome reference sequence (unpublished data), and comparing the miRNA sequences with the miRBase database, 179 known mature miRNAs and 100 novel miRNAs were identified. Expression analysis showed that, 165 miRNAs were differentially expressed between the ovary and testis. To validate the Illumina results, the expression patterns of the nine most differently expressed miRNAs (dre-miR-7a, dre-miR-7b, dre-miR-153a-3p, dre-miR-144-3p, dre-miR-301a, dre-miR-92a-3p, dre-miR-727-5p, Novel 124, and Novel 190, as well as those of five miRNAs that may relate to gonad development (dre-miR-143, dre-miR-101a, dre-miR-202-5p, dre-let-7c-5p, and dre-miR-181a-5p), were assayed with RT-qPCR. The expression trends for all the miRNAs validated with RT-qPCR were consistent with the next-generation sequencing data. The identification and characterization of the miRNAs differentially expressed between the ovary and the testis of the T. ovatus will increase our understanding of the role of miRNAs in gonad differentiation. These data also facilitate studies on miRNA regulation of teleost reproduction.
- Subjects :
- Male
Sex Differentiation
Gonad
Physiology
urologic and male genital diseases
Biochemistry
MiRBase
Transcriptome
03 medical and health sciences
Testis
Genetics
medicine
Animals
Sexual maturity
Molecular Biology
Trachinotus ovatus
030304 developmental biology
0303 health sciences
Sexual differentiation
biology
Gene Expression Profiling
Ovary
030305 genetics & heredity
Fishes
Gene Expression Regulation, Developmental
High-Throughput Nucleotide Sequencing
biology.organism_classification
MicroRNAs
medicine.anatomical_structure
Female
Development of the gonads
Reference genome
Subjects
Details
- ISSN :
- 1744117X
- Volume :
- 30
- Database :
- OpenAIRE
- Journal :
- Comparative Biochemistry and Physiology Part D: Genomics and Proteomics
- Accession number :
- edsair.doi.dedup.....956c28ea7e7c73143af2d4d27c71d1c7