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Biochemical characterization of pathogenic bacterial species using Raman spectroscopy and discrimination model based on selected spectral features
- Source :
- Lasers in Medical Science. 36:289-302
- Publication Year :
- 2020
- Publisher :
- Springer Science and Business Media LLC, 2020.
-
Abstract
- This study aimed to evaluate the differences in the Raman spectra of nine clinical species of bacteria isolated from infections (three Gram-positive and six Gram-negative species), correlating the spectra with the chemical composition of each species and to develop a classification model through discriminant analysis to categorize each bacterial strain using the peaks with the most significant differences. Bacteria were cultured in Mueller Hinton agar and a sample of biomass was harvested and placed in an aluminum sample holder. A total of 475 spectra from 115 different strains were obtained through a dispersive Raman spectrometer (830 nm) with exposure time of 50 s. The intensities of the peaks were evaluated by one-way analysis of variance (ANOVA) and the peaks with significant differences were related to the differences in the biochemical composition of the strains. Discriminant analysis based on quadratic distance applied to the peaks with the most significant differences and partial least squares applied to the whole spectrum showed 89.5% and 90.1% of global accuracy, respectively, for classification of the spectra in all the groups. Raman spectroscopy could be a promising technique to identify spectral differences related to the biochemical content of pathogenic microorganisms and to provide a faster diagnosis of infectious diseases.
- Subjects :
- Microorganism
Dermatology
Spectrum Analysis, Raman
Models, Biological
Vibration
030207 dermatology & venereal diseases
03 medical and health sciences
symbols.namesake
chemistry.chemical_compound
0302 clinical medicine
Partial least squares regression
Biochemical composition
Humans
Least-Squares Analysis
Chromatography
Bacteria
biology
Chemistry
Discriminant Analysis
030206 dentistry
Linear discriminant analysis
biology.organism_classification
Bacterial strain
Mueller-Hinton agar
symbols
Surgery
Raman spectroscopy
Subjects
Details
- ISSN :
- 1435604X and 02688921
- Volume :
- 36
- Database :
- OpenAIRE
- Journal :
- Lasers in Medical Science
- Accession number :
- edsair.doi.dedup.....9572972819efaf245b5a44f0553731dc