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Evaluation of the microbiota of primary endodontic infections using checkerboard DNA-DNA hybridization
- Source :
- Oral microbiology and immunology. 22(6)
- Publication Year :
- 2007
-
Abstract
- Background/aims The aim of this study was to evaluate the composition of the microbiota of primary endodontic infections in 111 selected cases of single-rooted teeth with necrotic pulp. Methods Samples were collected from the root canals using #15 Hedstroen-type files and two sterile paper points, which were introduced 1 mm short of the apical foramen. The presence, levels, and proportions of 40 different bacterial species in each sample were determined using DNA probes and checkerboard DNA-DNA hybridization techniques. Results The mean number of species per sample was 22. Enterococcus faecalis (89.3%), Campylobacter gracilis (89.3%), Leptotrichia buccalis (89.3%), Neisseria mucosa (87.5%), Prevotella melaninogenica (86.6%), Fusobacterium nucleatum ssp. vincentii (85.7%), Eubacterium saburreum (75.9%), Streptococcus anginosus (75%), and Veillonella parvula (74.1%) were the most prevalent species. The species found in highest mean counts (over 10(5)) were F. nucleatum ssp. vincentii (13.14 x 10(5)), E. saburreum (5.67 x 10(5)), E. faecalis (5.38 x 10(5)), N. mucosa (4.19 x 10(5)), V. parvula (3.63 x 10(5)), C. gracilis (3.46 x 10(5)), Treponema socranskii (3.34 x 10(5)), Porphyromonas endodontalis (2.96 x 10(5)), Porphyromonas gingivalis (2.85 x 10(5)), Micromonas micros (2.81 x 10(5)), Prevotella nigrescens (2.68 x 10(5)) and Fusobacterium nucleatum ssp. nucleatum (2.64 x 10(5)). Most of these species were also found in high proportions. Conclusions Our results suggest that several bacterial species considered to be oral pathogens seem to be implicated in the etiology of primary endodontic infections.
- Subjects :
- Microbiology (medical)
Adult
DNA, Bacterial
Male
Porphyromonas endodontalis
Adolescent
Immunology
Veillonella
Colony Count, Microbial
Microbiology
Enterococcus faecalis
Veillonella parvula
Prevotella melaninogenica
Prevotella nigrescens
Dental Pulp Necrosis
Humans
Treponema
General Dentistry
Gram-Positive Bacterial Infections
Leptotrichia
Aged
Leptotrichia buccalis
biology
Fusobacterium nucleatum
Eubacterium
Peptostreptococcus
Neisseria mucosa
Nucleic Acid Hybridization
Campylobacter
Middle Aged
biology.organism_classification
Streptococcus anginosus
Female
Dental Pulp Cavity
DNA Probes
Gram-Negative Bacterial Infections
Porphyromonas gingivalis
Campylobacter gracilis
Subjects
Details
- ISSN :
- 09020055
- Volume :
- 22
- Issue :
- 6
- Database :
- OpenAIRE
- Journal :
- Oral microbiology and immunology
- Accession number :
- edsair.doi.dedup.....958635dcea5bb262c44386a1a429fd4b