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Human myelin basic protein specific T cell lines display differential cytotoxicity against astrocytes, but are consistently cytotoxic against monocytes
- Source :
- Journal of Neuroimmunology. 88:99-104
- Publication Year :
- 1998
- Publisher :
- Elsevier BV, 1998.
-
Abstract
- In experimental allergic encephalomyelitis (EAE) myelin basic protein (MBP) specific T cells differ in their encephalitogenic potential. To investigate the functional diversity of human MBP specific T cell lines, we analysed their cytotoxic activity against human astrocytes and monocytes. Five out of 14 MBP specific T cell lines killed astrocytes in the presence of MBP. Nevertheless, all lines lysed blood derived monocytes. T cell lines that lysed astrocytes efficiently in the presence of MBP, recognized peptide aa 80-99/86-105 in context with HLA-DRB5 * 0101, peptide aa 50-69/61-83 in context with HLA-DRB1 * 1501 and peptides aa 139-153, and aa 148-162 in context with HLA-DRB1 * 0101. There was no correlation of MBP-mediated lysis of astrocytes with TCR-Vbeta usage, HLA-restriction and the production of tumor-necrosis-factor-alpha (TNF-alpha), lymphotoxin (LT) and interferon-gamma (IFN-gamma). Different lysis of astrocytes, however, revealed a functional heterogeneity of MBP specific T cells, which was not observed by using monocytes as targets.
- Subjects :
- Receptors, Antigen, T-Cell, alpha-beta
T cell
Immunology
Context (language use)
Human leukocyte antigen
Monocytes
Cell Line
03 medical and health sciences
0302 clinical medicine
HLA Antigens
medicine
Humans
Immunology and Allergy
Cytotoxic T cell
030304 developmental biology
0303 health sciences
biology
Myelin Basic Protein
Molecular biology
Myelin basic protein
medicine.anatomical_structure
Lymphotoxin
Neurology
Cell culture
Astrocytes
biology.protein
Cytokines
Neurology (clinical)
Peptides
030217 neurology & neurosurgery
T-Lymphocytes, Cytotoxic
Astrocyte
Subjects
Details
- ISSN :
- 01655728
- Volume :
- 88
- Database :
- OpenAIRE
- Journal :
- Journal of Neuroimmunology
- Accession number :
- edsair.doi.dedup.....95e40507dd15ec8d7aba785fd11c5821