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Identification of the metal ion binding site on an RNA motif from hammerhead ribozymes using (15)N NMR spectroscopy
- Source :
- Journal of the American Chemical Society. 124(17)
- Publication Year :
- 2002
-
Abstract
- An RNA oligomer, r(GGACGAGUCC), which mimics the metal ion-binding motif of hammerhead ribozymes, was shown to fold by itself into a conformation possessing a metal ion binding property which is similar to that of the intact ribozyme (Tanaka, et al. J. Am. Chem. Soc. 2000, 122, 11303-11310). To determine the metal ion-binding site of this motif at an atomic level, we synthesized a series of RNA oligomers which were selectively labeled with a (15)N-labeled guanosine at each of the four guanosine residues. The (15)N-chemical shift perturbation with Cd(II) ions by one-dimensional (1D) (15)N NMR spectra showed that the chemical shift of the N7 of the G7 residue, N7/G7, in the metal ion-binding motif was specifically perturbed. This is the first experimental evidence to prove that the N7/G7 binds with a Cd(II) ion.
- Subjects :
- Stereochemistry
Cations, Divalent
Guanosine
Biochemistry
Oligomer
Catalysis
chemistry.chemical_compound
Colloid and Surface Chemistry
Ion binding
RNA, Catalytic
Binding site
Nuclear Magnetic Resonance, Biomolecular
Binding Sites
biology
Nitrogen Isotopes
Molecular Mimicry
Ribozyme
RNA
General Chemistry
Nuclear magnetic resonance spectroscopy
chemistry
biology.protein
Nucleic Acid Conformation
Hairpin ribozyme
Cadmium
Subjects
Details
- ISSN :
- 00027863
- Volume :
- 124
- Issue :
- 17
- Database :
- OpenAIRE
- Journal :
- Journal of the American Chemical Society
- Accession number :
- edsair.doi.dedup.....95e908e0a5d3169cff44c484a3abe778