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Citric acid mitigates soybean meal induced inflammatory response and tight junction disruption by altering TLR signal transduction in the intestine of turbot, Scophthalmus maximus L
- Source :
- Fish & Shellfish Immunology. 92:181-187
- Publication Year :
- 2019
- Publisher :
- Elsevier BV, 2019.
-
Abstract
- A 12-week feeding trial was conducted to investigate the effect of citric acid on the involvement of TLRs in the soybean meal induced inflammatory response and tight junction disruption in the distal intestine of juvenile turbot (Scophthalmus maximus L.). Four isonitrogenous and isolipidic practical diets were formulated: fish meal-based diet (FM); 40% fish meal protein in FM replaced with soybean meal protein (SBM); SBM + 1.5% citric acid and SBM + 3% citric acid. Compared to the FM, diet SBM significantly increased the gene expression of TLRs (TLR2, TLR3, TLR5b, TLR9, TLR21, TLR22) and MyD88, as well as TLR related molecules (NF-κB, IRF-3, p38 and JNK), which were remarkably reduced by dietary citric acid. Similarly, citric acid supplementation in SBM markedly depressed gene expression of pro-inflammatory cytokines (TNF-α and IFN-γ) and pore-forming tight junction protein Claudin-7, and enhanced gene expression of the anti-inflammatory cytokine TGF-β1 and TJ proteins related to the decrease in paracellular permeability (Claudin-3, Claudin-4, Occludin, Tricellulin and ZO-1). Compared to the SBM, the concentration of IgM and C4 in serum was significantly reduced by dietary citric acid. In brief, dietary citric acid could synchronously inhibit TLRs-dependent inflammatory response regulated by NF-κB and IRF3, as well as cause TLRs-dependent tight junction disruption modulated by p38 and JNK. Therefore, citric acid could function on mitigating soybean meal induced enteropathy in the distal intestine of juvenile turbot.
- Subjects :
- Fish Proteins
0301 basic medicine
medicine.medical_specialty
Soybean meal
Aquatic Science
Occludin
Citric Acid
Tight Junctions
Random Allocation
03 medical and health sciences
chemistry.chemical_compound
Fish meal
Internal medicine
Gene expression
medicine
Animals
Environmental Chemistry
Inflammation
Tight junction
biology
Toll-Like Receptors
04 agricultural and veterinary sciences
General Medicine
biology.organism_classification
Animal Feed
Diet
Intestines
Turbot
TLR2
030104 developmental biology
Endocrinology
chemistry
Dietary Supplements
Flatfishes
040102 fisheries
0401 agriculture, forestry, and fisheries
Soybeans
Citric acid
Signal Transduction
Subjects
Details
- ISSN :
- 10504648
- Volume :
- 92
- Database :
- OpenAIRE
- Journal :
- Fish & Shellfish Immunology
- Accession number :
- edsair.doi.dedup.....95e9909f39903ea16c35e4adcf25215b